黏萼蝇子草蜕皮甾酮类成分的分离与鉴定

Isolation and identification of ecdysterones from root of Silene viscidula Franch.

目的对黏萼蝇子草根的化学成分进行研究。方法采用反复硅胶柱色谱、Sephadex LH-20柱色谱和反相半微量制备型高效液相色谱分离纯化,根据ESI-MS、1H-NMR和13C-NMR等谱学数据进行结构鉴定。结果从黏萼蝇子草根的乙醇提取物分离得到5个蜕皮甾酮类化合物,分别鉴定为20-羟基蜕皮激素(20-hydroxyecdysone,1)、1-epi-integristerone A(2)、abutasterone(3)、旌节花甾酮A(stachysterone A,4)和15-羟基旌节花甾酮A(15-hydroxystachysterone A,5);另外得到胡萝卜苷(daucosterol,6)和β-谷甾醇(β-sitosterol,7)2个甾醇类化合物。结论化合物2-5为蝇子草属植物中首次分离的化合物。

Objective To study the constituents of the extract from the root of Silene viscidula Franch.Methods The chemical constituents were isolated by silica gel column,open ODS column,Sephadex LH-20 column chromatography,and semi-preparative HPLC.Chemical characters and spectroscopic analysis were employed for the structural identification.Results Five ecdysterone compounds were obtained and their structures were identified as 20-hydroxyecdysone（1）,1-epi-integristerone A（2）,abutasterone（3）,stachysterone（4）and 15-hydroxystachysterone A（5）,along with two sterol compounds daucosterol（6）and β-sitosterol（7）.Conclusions Compounds 25 are obtained from the genus Silene for the first time.