RP-HPLC法同时测定红景天口服液中红景天苷和酪醇的含量

Simultaneous determination of salidroside and tyrosol in Hongjingtian oral solution by RP-HPLC

目的建立反相高效液相色谱法同时测定红景天口服液中红景天苷和酪醇的含量。方法采用Kromasil C18柱（250 mm×4.6 mm,5μm）;甲醇-水（体积比为20∶80）为流动相;柱温：30℃;检测波长：275 nm;流速：1.0 mL.min-1。结果红景天苷和酪醇分别在0.048-0.960 g.L-1（r=0.999 9,n=6）和0.013-0.390 g.L-1（r=1.000 0,n=6）内线性关系良好;平均回收率（n=9）分别为95.5%（RSD=1.3%）和95.2%（RSD=1.6%）。结论本方法可用于红景天口服液的质量控制。

Objective To establish an RP-HPLC method for determination of salidroside and tyrosol in Hongjingtian oral solution（traditional Chinese medicines）.Methods The chromatographic method was carried out on Kromasil C18 column（250 mm×4.6 mm,5 μm）using methanol-water（V∶V=20∶80）as the mobile phase,with the column temperature at 30 ℃.The detection wavelength was set at 275 nm,and the flow rate was 1.0 mL·min-1.Results The linear ranges were 0.048-0.96 g·L-1（r=0.999 9,n=6）for salidroside and 0.013-0.39 g·L-1（r=1.000 0,n=6）for tyrosol,respectively.The average recoveries（n=9）were 92.5%（RSD=1.3%）and 95.2%（RSD=1.6%）,respectively.Conclusions The method can be used for the quality control of Hongjingtian oral solution.