鱼腥草注射液中过敏原成分

Allergen component of Houttuynia cordata injection

目的探索鱼腥草注射液中的过敏原成分及其致敏机理。方法分别用鱼腥草注射液、鱼腥草蒸馏液、吐温80、阴性对照物和阳性对照物对豚鼠和Brown Norway(BN)大鼠进行全身主动过敏试验,并用吐温80首次静脉注射豚鼠和BN大鼠进行类过敏试验。观察其行为学反应并检测动物血清中组胺、IL-4、IgE、IgG和IgM的水平。结果阳性对照组、鱼腥草注射液组、吐温80组和鱼腥草蒸馏液组动物都观察到不同程度的行为学过敏反应,且动物血清中的组胺、IgE和IL-4水平,以及阳性对照组和吐温80组的BN大鼠血清中IgM水平与阴性对照组相比显著性升高,IgG、IgM及鱼腥草蒸馏液组BN大鼠血清中组胺和IL-4水平无显著变化。吐温80类过敏试验组,动物的行为学及血清中各生化指标均未见显著变化。结论吐温80为鱼腥草注射液中的主要过敏原,可以引起豚鼠和BN大鼠发生Ⅰ型全身主动过敏反应,而不引起豚鼠和BN大鼠发生类过敏反应。鱼腥草蒸馏液中含有其他引起过敏反应的过敏原。

Objective To explore the allergen ingredients of Houttuynia cordata injection and the sensitization mechanism.Methods Active systemic anaphylaxis test was carried out on Guinea pigs and the BN rats with Houttuynia cordata injection,Houttuynia cordata distillate,polysorbate 80,negative control and positive control,and anaphylactoid reactions were carried out by intravenous polysorbate 80 to guinea pigs and BN rats for the first time.The anaphylaxis situations of guinea pigs and BN rats were observed,and the concentrationns of histamine,IgE,IgM,IgG and IL-4 in serum were measured with ELISA.Results Positive control group,Houttuynia cordata injection group,polysorbate 80 group and Houttuynia cordata distillate group animals had been observed to have various degrees of behavioral hypersensitivity reactions,histamine,meanwhile IgE and IL-4 levels in these groups′serum,and IgM in BN rats′serum of both positive control group and polysorbate 80 group,were significantly mcreased compared with the negative control group,IgG,IgM levels and the histamine,IL-4 levels in in BN rats′serum of Houttuynia cordata distillate group were not significantly changed.In polysorbate 80 anaphylactoid reactions group,both animal behavior observation and serum biochemical parameters were not found in significant changes.Conclusions This study shows that polysorbate 80 is the main allergens in Houttuynia cordata injection,which can cause typeⅠactive systemic allergic reactions on guinea pigs and BN rats,rather than anaphylactoid reactions.Houttuynia cordata distillate contains other allergens that cause allergic reactions.