粉防己碱对吗啡在离体豚鼠回肠中依赖性的作用(英文)

Effect of tetrandrine on morphine dependence in isolated guinea pig ileum

目的:研究粉防己碱(Tet)和尼莫地平(Nim)对吗啡在离体豚鼠回肠中戒断性反应的影响。方法:戒断性收缩由纳洛酮(1μmol·L^(-1))加入已在含吗啡(3μmol·L^(-1))的37.5℃ Krebs液中孵育4h的离体豚鼠回肠或加入从吗啡依赖豚鼠中取得的回肠引起。结果:离体豚鼠回肠在含吗啡的Krebs液中孵育4h,给Nim(0.01、0.05和0.1μmol·L^(-1))或Tet(1、10和50μmol·L^(-1))抑制其戒断性收缩。Nim和Tet体内或体外给药都能抑制吗啡依赖豚鼠离体回肠的戒断性收缩。结论:钙拮抗剂Nim和Tet抑制吗啡在离体豚鼠回肠的戒断性收缩。

AIM: To evaluate the effects of tetrandrine (Tet) and nimodipine (Nim) on the morphine (Mor) withdrawal response in the isolated guinea pig ileum. METHODS: The withdrawal contracture was elicited by addition of naloxone (Nal) (1 μmol·L-1) to the isolated naive ileum incubated with Mor (3 μmol·L-1) at 37.5 ℃ for 4 h or to the ileum obtained from Mor-dependent guinea pig. RESULTS: When Nim (0.01, 0.05, and 0.1 μmol·L-1) or Tet (1, 10, and 50 μmol·L-1) was added 1 min before Nal in the naive ilea bathed in Krebs solution containing Mor, or when the ilea from Mor-dependent guinea pigs were incubated with Nim (0.01, 0.05, and 0.1 μmol·L-1) or Tet (1, 10, and 50 μmol·L-1) for 15 min, or when Nim (5 and 10 mg·kg-1, ip) or Tet (15 and 30 mg·kg-1, ip) was administered in vivo to Mor-dependent guinea pigs, the Nal-precipitated withdrawal contracture was significantly decreased in a dose-dependent manner. CONCLUSION: Tet and Nim, Ca2+ channel blockers, could inhibit the Nal-precipitated Mor withdrawal response in the isolated guinea pig ileum.