甜菜多糖提取条件研究

Study on Extraction Technology of Polysaccharides from Beta Vulgaris

在单因素试验基础上设计L9(33)正交试验,研究不同料液比、提取时间、提取温度对甜菜多糖得率的影响,筛选出最佳提取工艺,纯化方法采用Sevag法除蛋白。结果表明:影响甜菜多糖提取率的主次关系依次是提取时间、料液比、提取温度,甜菜多糖最佳提取工艺为:提取温度80℃,浸提时间2 h,固液比1∶90。在此工艺条件下,甜菜多糖的得率为3.7%。

Using the methods of single-factor test and orthogonal design test of L9（33）,the effects of various factors such as the ratio of sample to water,the extraction time and temperature on the extraction of polysaccharides in beta vulgaris were investigated and the optimal conditions were concluded.The polysaccharides were purified by Sevag method.The results showed that the main factors from the most important to the less affecting the extraction efficiency of polysaccharides are as following： extraction time,ratio of sample to water and extraction temperature.The optimal extracting conditions are established as following： extraction time 2 h,ratio of sample to water 1∶90 and extraction temperature 80℃.Under the optimized parameters,the extracting rate of polysaccharides in beta vulgaris is 3.7%.