北京市西城区2009年-2010年B型流感监测分析

Analysis on surveillance influenza B in Xicheng district,Beijing,2009-2010

目的：了解北京市西城区B型流感的流行状况,为制定预防控制措施提供依据。方法：由两家哨点医院每周随机采集流感样病例的咽拭子共40~60份,提取病毒RNA,应用real-time PCR法和MDCK细胞分离法进行检测。结果：2077件流感样标本中有373件real-time PCR检测B型流感阳性,总阳性率为18.0%。阳性率最高的是2010年1月,为81.6%。西城区于2010年1月-2010年2月出现一次B型流感小规模社区暴发,流行呈波浪进程,于2010年第1周至第8周出现一次流行高峰。儿童和老年人B型流感阳性率比较高。373件real-time PCR法阳性标本经MDCK细胞培养后,共有91件阳性,阳性率为24.4%（91/373）。结论：应加强监测管理,并尽快采取预防控制措施以保护广大人群身体健康。

Objective：To understand the epidemic status of influenza B in Xicheng district of Beijing to provide evidences for formulating measures of its prevention and control.Methods： Forty to sixty throat swabs of influenza-like patient were randomly collected from two hospitals.The viral RNA was extracted,identified by real-time PCR assay and isolated with MDCK cells.Results： There were 373 samples to be positive of 2077 samples of throat swabs of influenza-like patient,which were identified by real-time PCR that the total positive rate were 18.0%.The highest positive rate is 81.6% in January 2010.There was a little scale community influenza B outbreak in Xicheng district,Beijing.It was a wave epidemic process.And there was a epidemic peak during the first week to the eighth week in 2010.The positive rate of children and the old were high.There were 91 samples to be positive in virus isolation with MDCK cells of 373 positive samples which were identified by real-time PCR assay.The virus isolation positive rate was 24.4%（91/373）.Conclusion： Management of menitoring should be strengthened,and necessary measure should be taken promptly to protect pubic health.