疏肝补肾方药对疲劳大鼠海马CA1区中蛋白激酶C表达的影响

Effects of Soothing Liver and Tonifying Kidney Formula on Protein Kinase C Level in the Hippocampal CA1 Region of Fatigue Rats

目的:研究疏肝补肾方药对于疲劳大鼠海马CA1区中蛋白激酶C(PKC)水平的变化。方法:成年雄性Spargue-Dawley大鼠36只,随机分为模型组(MG)、对照组(CG)和疏肝补肾组(LK)。应用大鼠游泳运动结合睡眠剥夺建立疲劳大鼠复合模型,以Y迷宫实验评定其学习记忆能力。取大鼠海马CA1区以Western Blot定量检测,并以Real-time PCR技术分析海马CA1区PKC的mRNA表达。结果:Y迷宫实验显示,用药后大鼠的学习和记忆能力优于模型组,疏肝补肾组大鼠在正确反应率和达标所需训练次数皆与模型组有差异(P<0.01或0.05)。WesternBlot结果显示,模型组PKC的蛋白表达明显低于对照组(P<0.05),疏肝补肾组高于模型组(P<0.01)。Real-time PCR结果显示,在mRNA水平上模型组及疏肝补肾组与对照组比较都有明显差异(P<0.01),疏肝补肾组PKCmRNA表达高于模型组(P<0.01)。结论:疲劳引起大鼠海马CA1区的PKC水平下调,而使用疏肝补肾方药可改善之,提示可能藉由疏肝补肾法对抗疲劳引起的学习记忆损伤。

Objective ： To study the effects of soothing liver and tonifying kidney formula on the protein kinase C （PKC） level in the hippocampal CA1 region of fatigue rats. Method： Thirty-six male adult Spargue-Dawley rats were randomly divided into three groups, namely model group （ MG, n = 12 ） , control group （ CG, n = 12 ） and soothing liver and tonifying kidney formula group （ LK, n = 12 ）. The fatigue rats＇ model was established by swimming exercise combined with sleep deprivation. Y maze was used to test the memo- ry and learning ability of the rats. The PKC expression in the hippocampal CA1 region was tested by Western Blot and Real-time PCR. Result ： Y maze test showed the memory and learning ability of rats in LK were better than those in MG. The correct response rate and standard required training times between the two groups had significant difference （P 〈 0. 01 and P 〈 0. 05, respectively）. The expres- sion of PKC in MG was lower than that in CG （P 〈 0. 05 ）, while that in LK was higher than that in MG （P 〈 0.01 ）. There was signifi- cant difference in PKC mRNA expression of MG and LK when compared with that of CG （P 〈0. 01 ） ,which was highest in LK （P 〈 0. 01 ）. Conclusion： Fatigue can cause the decrease of PKC level in the hippocampal CA1 region,and can be ameliorated by soothing liver and tonifying kidney formula, suggesting that the damage of learning and memory ability brought by fatigue can be improved by the formula as well.