常见伴生植物对药用植物金毛狗Cibotium barometz孢子萌发和配体子体发育的化感作用研究

Allelopathic effects of companion species on spore germination and gametophyte development in Cibotium barometz

目的:通过伴生种镰羽复叶耳蕨Arachniodes falcate和山姜Alpinia japonica水浸出液对金毛狗孢子萌发和配子体发育作用的研究,弄清伴生种与金毛狗的化感作用。方法:将金毛狗成熟孢子分别接种在含有不同浓度的2种伴生植物浸出液的MS培养基上,利用显微技术,观察记录孢子萌发和配子体发育过程。结果:金毛狗孢子萌发延迟,假根伸长受抑制;对原叶体形成和性别分化均具有明显的延迟和抑制作用,浓度越高抑制作用越明显。结论:2种伴生植物对金毛狗孢子萌发和配子体发育化感作用显著,这可能对金毛狗孢子体产生以及种群壮大具有较大的影响。

Objective： To study the relationship between Cibotium barometz and its companion species, Arachniodesfalcate and Alpinia japonica, we used aqueous leachates of the two companions to deal with C. barometz spores. Method： Spores of C. barometz were translated on MS culture which contained different concentration of aqueous extracts of the two companions, the germination and gametophyte development were observed and recorded. Result： All extracts inhibited and delayed the C. barometz spores germination and rhizoid elongation was inhibited. It also had obvious inhibition to the prothallus formation and sexual differentiation. And the higher concentration, the more obvious inhibition of aqueous extracts of the two companion species. Conclusion： The two companion species have allelopathic effects on the spore germination and gametophyte development of C. barometz. And it may have an influence on sporogon ontogenesis and the population expansion.