桦褐孔菌乙醇提取物在小鼠哮喘模型中对p38 MAPK信号通路的影响

Protective effects and mechanism of Inonotus obliquus on asthmatic mice

目的:探讨桦褐孔菌乙醇提取物对哮喘小鼠气道炎症和Thl/Th2类细胞因子变化的影响及其可能机制。方法:雄性BALB/c小鼠32只随机分成4组,即正常对照组、哮喘模型组和桦褐孔菌乙醇提取物低、高剂量干预组。分别采用酶联免疫吸附法(ELISA)和蛋白质印迹检测支气管肺泡灌洗液(BALF)中IL-4,IL-5,IL-13,IFN-γ含量以及肺组织中磷酸化p38MAPK的变化,并观察BALF中炎症细胞和肺组织病理学改变。结果:哮喘模型组小鼠BALF中炎症细胞计数,IL-4,IL-5,IL-13水平和IFN-γ水平降低;肺组织中磷酸化p38 MAPK表达水平升高,与正常组比较差异显著(P<0.05)。桦褐孔菌乙醇提取物低、高剂量干预组小鼠BALF中炎症细胞计数,IL-4,IL-5,IL-13水平和肺组织中磷酸化p38MAPK表达水平均明显降低,IFN-γ水平明显上升,与哮喘模型组比较均具有显著差异(P<0.05)。桦褐孔菌乙醇提取物处理能明显减轻哮喘小鼠肺组织病理学改变,桦褐孔菌低、高剂量干预组之间比较无显著差异。结论:p38 MAPK可能参与了支气管哮喘的发病过程。桦褐孔菌对哮喘小鼠具有保护作用,其机制可能与抑制p38 MAPK磷酸化、调节IL-4/IFN-γ平衡失调、减轻炎症细胞浸润有关。

Objective： To explore the protective effects and mechanism of ethanol extract of Inonotus obliquus （EEIO） injection on asthmatic mice. Method： OVA was injected intraperitoneally and inhaled to produce the asthmatic model. Thirty two mice were randomly divided into four groups ： control group, asthma group and 1. obliquus groups of high and low dose. The concentrations of IL- 4,IL-5 ,IL-13 and IFN-γ in BALF, the phosphor-p38 MAPK in lung tissues were respectively measured by ELISA and Western blotting. The number of inflammatory cells in BALF and histopathology changes were observed. Result： In asthmatic group,the number of inflammatory cells and the concentrations of IL-4, IL-5, IL-13 in BALF and phospho-p38 MAPK in lung tissue were higher, while IFN-γ were lower than those in normal control mice （ P 〈 0. 05 ）. In 1. obliquus group, the number of inflammatory cells, the concentrations of IL-4,IL-5 ,IL-13 in BALF and phosphor-p38 MAPK in lung tissue were lower, but were higher than those in normal control mice （ P 〈 0.05 ）, and histropathology damage was alleviated significantly. There was no significant difference observed among the efficacies in the I. obliquus groups of high and low dose. Conclusion： p38 MAPK may play a role in pathological process of asthma. I. obliquus effectively treats asthma by inhibiting the expression of phosphor-p38 MAPK, correcting the unbalance of IFN-3,/IL-γ and decreasing the number of inflammatory cells.