通过酵母双杂交筛选水稻类受体激酶CR4的胞外结合蛋白

Screening of Extracellular Binding Proteins of Rice Receptor-like Kinase CR4 by the Yeast Two-hybrid

[目的]寻找CR4的胞外结合蛋白。[方法]采用OsCR4的胞外域作为诱饵蛋白,酵母双杂交筛选14 d的小苗cDNA文库。[结果]得到了许多蛋白,包括一个过氧化物B(D26484)、甲硫氨酸硫氧还原酶(ABF96078)和一个未知功能的蛋白。[结论]为研究CR4的信号转导机制提供了理论依据。

[Objective] The research aimed to find the extracellular binding proteins of CR4.[Method] The extracellular region of OsCR4 was as the bait protein,and the yeast two-hybrid was used to screen the seedling cDNA library which was cultivated 14 d.[Result] A lot of proteins which included a peroxid B（D26484）,a methionine thioredoxin reductase（ABF96078） and an unknown function protein were gained.[Conclusion]It provided the theory basis for studying the signal transduction mechanism of CR4.