不同品种金银花叶片中脯氨酸对盐胁迫的响应

Effects of Salt Stress on the Contents of Proline in Different Varieties of Lonicera japonica Thunb.

[目的]研究不同品种金银花(Lonicera japonica Thunb)叶片中脯氨酸对盐胁迫的响应。[方法]采用5个不同浓度(0、100、200、400和600 mmol/L)的NaCl溶液对金银花苗进行盐分胁迫试验,以叶片中脯氨酸含量作为评价指标研究不同品种金银花耐盐碱的特性。[结果]短期盐处理后,树型金银花叶片中脯氨酸含量随NaCl浓度的增加急剧增加;藤状金银花叶片在不同浓度之间无显著差异。但随着胁迫时间的加长,藤状金银花叶片中脯氨酸含量随Nacl浓度的增加而显著增加。2个品种的的金银花脯氨酸含量均为NaCl浓度400 mmol/L处理的值最大。[结论]与藤状金银花比较,树型金银花叶片中脯氨酸对盐胁迫更敏感,表现出较强的渗透调节能力。

[Objective] To study the effects of salt stress on the contents of proline in the leaves from different varieties of Lonicera japonica Thunb..[Method] The flower seedlings of Lonicera japonica Thunb.were treated with different concentrations of NaCl solution（0,100,200,400 and 600 mmol/L）,and the proline content can be used as indicator for evaluating the salt tolerance of Lonicera japonica Thunb..[Result] After short treatment,the content of proline in the leaves of treelike Lonicera japonica Thunb.increased dramatically with the concentration of NaCl increased;although there were no significant difference among the lianoid Lonicera japonica Thunb.groups,the content of proline in the leaves of lianoid Lonicera japonica Thunb.increased dramatically with the concentration of NaCl increased as time went by.The contents of proline in treelike and lianoid Lonicera japonica Thunb.achieved the highest level when the concentration of NaCl was 400 mmol/L.[Conclusion] The proline in the leaves of treelike Lonicera japonica Thunb.was more sensitive to salt stress and showed stronger osmotic regulation ability.