摘要
[目的]研究Cu2+、Cd2+、Cr6+对斑马鱼抗氧化酶活性影响,探讨重金属对斑马鱼毒性大小。[方法]以斑马鱼为模式生物,采用半静态法对斑马鱼进行驯养和试验,测定Cu2+、Cd2+和Cr6+对超氧化物歧化酶(SOD)、过氧化物酶(POD)和过氧化氢酶(CAT)活性影响。[结果]Cu2+暴露1d胁迫斑马鱼SOD和CAT为诱导效应,胁迫POD为抑制-诱导-抑制效应,Cu2+暴露7 d胁迫斑马鱼SOD为诱导效应,胁迫POD和CAT为抑制效应;Cd2+和Cr6+暴露1 d和7 d胁迫斑马鱼SOD和CAT均为诱导效应,胁迫POD为抑制效应。且暴露1 d和暴露7 d酶活性变化均显著。[结论]Cu2+、Cd2+和Cr6+单一胁迫斑马鱼抗氧化系统酶活性变化显著,在短时间和低浓度暴露下,Cr6+毒性作用最显著;在短时间中、高浓度暴露下,Cd2+毒性作用最显著;长时间暴露时,Cu2+毒性作用最强,可为重金属污染对水生生物毒性机理研究提供参考。
[Objective] The aim was to study the effect of Cu2+,Cd2+and Cr6+ on the anti-oxidative enzyme activities in Danio rerio,and discuss the toxicity effect of heavy metal on Danio rerio.[Method] Danio rerio was viewed as model organism,semi-static toxicity test was used in domestication and experiments of Danio rerio,the effects of Cu2+,Cd2+and Cr6+ on superoxide dismutase(SOD),peroxidase(POD) and catalase(CAT) of it were discussed.[Result] At 1 d,effects of Cu2+ stress on SOD in Danio rerio was induction effect,and the stress on POD was inhibition-induction-inhibition effect,and stress on CAT was induction effect.At 7 d,effects of Cu2+ stress on SOD in Danio rerio was induction effect,and stress on POD was inhibition effect,and stress on CAT was inhibition effect.At 1 and 7 d,effects of Cd2+ stress on SOD in Danio rerio were all induction effect,and stress on POD and CAT were all inhibition effect.Moreover,the enzyme activities were changed markedly significantly at from 1 to 7d.[Conclusion] Cu2+,Cd2+and Cr6+ single stress on the anti-oxidative enzyme activities in Danio rerio were changed markedly.In short time and low-concentration exposure,Cr6+ toxicity was the most significantly;in short time and high-concentration exposure,Cd2+ toxicity was the most the most significantly;in long time exposure,Cu2+ toxicity was the most significantly.The study could provide references for heavy metal pollution on the aquatic organism toxicity mechanism.
出处
《安徽农业科学》
CAS
北大核心
2011年第8期4739-4741,共3页
Journal of Anhui Agricultural Sciences
基金
山东省社会科学规划项目(03CJZ03)
山东省高等学校实验技术研究项目(2005-188)