Cu^(2+)、Cd^(2+)、Cr^(6+)胁迫对斑马鱼抗氧化酶活性的影响

Effect of Cu^(2+),Cd^(2+) and Cr^(6+) Stress on the Anti-oxidative Enzyme Activities in Danio rerio

[目的]研究Cu2+、Cd2+、Cr6+对斑马鱼抗氧化酶活性影响,探讨重金属对斑马鱼毒性大小。[方法]以斑马鱼为模式生物,采用半静态法对斑马鱼进行驯养和试验,测定Cu2+、Cd2+和Cr6+对超氧化物歧化酶(SOD)、过氧化物酶(POD)和过氧化氢酶(CAT)活性影响。[结果]Cu2+暴露1d胁迫斑马鱼SOD和CAT为诱导效应,胁迫POD为抑制-诱导-抑制效应,Cu2+暴露7 d胁迫斑马鱼SOD为诱导效应,胁迫POD和CAT为抑制效应;Cd2+和Cr6+暴露1 d和7 d胁迫斑马鱼SOD和CAT均为诱导效应,胁迫POD为抑制效应。且暴露1 d和暴露7 d酶活性变化均显著。[结论]Cu2+、Cd2+和Cr6+单一胁迫斑马鱼抗氧化系统酶活性变化显著,在短时间和低浓度暴露下,Cr6+毒性作用最显著;在短时间中、高浓度暴露下,Cd2+毒性作用最显著;长时间暴露时,Cu2+毒性作用最强,可为重金属污染对水生生物毒性机理研究提供参考。

[Objective] The aim was to study the effect of Cu2＋,Cd2＋and Cr6＋ on the anti-oxidative enzyme activities in Danio rerio,and discuss the toxicity effect of heavy metal on Danio rerio.[Method] Danio rerio was viewed as model organism,semi-static toxicity test was used in domestication and experiments of Danio rerio,the effects of Cu2＋,Cd2＋and Cr6＋ on superoxide dismutase（SOD）,peroxidase（POD） and catalase（CAT） of it were discussed.[Result] At 1 d,effects of Cu2＋ stress on SOD in Danio rerio was induction effect,and the stress on POD was inhibition-induction-inhibition effect,and stress on CAT was induction effect.At 7 d,effects of Cu2＋ stress on SOD in Danio rerio was induction effect,and stress on POD was inhibition effect,and stress on CAT was inhibition effect.At 1 and 7 d,effects of Cd2＋ stress on SOD in Danio rerio were all induction effect,and stress on POD and CAT were all inhibition effect.Moreover,the enzyme activities were changed markedly significantly at from 1 to 7d.[Conclusion] Cu2＋,Cd2＋and Cr6＋ single stress on the anti-oxidative enzyme activities in Danio rerio were changed markedly.In short time and low-concentration exposure,Cr6＋ toxicity was the most significantly;in short time and high-concentration exposure,Cd2＋ toxicity was the most the most significantly;in long time exposure,Cu2＋ toxicity was the most significantly.The study could provide references for heavy metal pollution on the aquatic organism toxicity mechanism.