染料木素对心肌缺血大鼠的血流动力学影响

Effect of Genistein on Hemodynamics of Myocardial Ischemia Rats

[目的]研究染料木素对大鼠心肌缺血的保护作用,观察染料木素对心肌缺血大鼠的血流动力学各项参数的影响。[方法]采用结扎左冠状动脉前降支致急性心肌梗死模型,将心肌梗死模型大鼠随机分为:心肌缺血模型组,假手术组,染料木素高、中、低剂量组,丹参对照组,测左心室等容收缩期室内压最大上升速率(+dp/dtmax)、左心室等容舒张期室内压最大下降速率(-dp/dtmax);运用N-BT染色法测定各组心肌缺血梗死面积。[结果]染料木素可明显增加心肌缺血大鼠LVSP、±dp/dt max,降低LVEDP,与模型组相比有显著性差异(P<0.05);染料木素可以明显减少结扎大鼠冠脉所致大鼠心肌缺血所引起的心肌梗死面积,与模型组相比有显著性差异(P<0.05)。[结论]染料木素可提高大鼠心肌缺血心室壁顺应性,改善心脏功能,降低心肌缺血心肌梗死面积。

[Objective]The aim was to study the protection of genistein for myocardial ischemia rats and to observe the effect of genistein on the hemodynamic parameters in myocardial ischemia rats.[Method]To establish acute myocardial infarction（AMI） model by ligating left anterior descending coronary artery.The myocardial infarction model rats were randomly divided into： myocardial ischemia model group,sham operation group,genistein high,medium and low dose group,Salvia miltiorrhiza Bunge control group.To measure the maximal rise rate（＋dp/dtmax） of left ventricular isovolumic contraction ventricular pressure and the maximum rate descent（-dp/dtmax） of left ventricular isovolumic relaxation ventricular pressure in the same time.To determine myocardial infarction area in each group based on N-BT staining method.[Results] Genistein significantly increased LVSP,± dp/dt max and decreased LVEDP of myocardial ischemia rats.There was significant difference（P0.05） compared with model group.Genistein can reduce myocardial infarction size of myocardial ischemia rats caused by ligating coronary artery.There was significant difference（P0.05） compared with model group.[Conclusion]Genistein can improve ventricular wall compliance of myocardial ischemia rats,improve cardiac function and reduce myocardial infarction size of myocardial ischemia rats.