大孔树脂分离制备麦芽低聚糖

Separation and Preparation of Malto-oligosaccharide using Macroporous Resin

[目的]介绍大孔树脂吸附法制备麦芽低聚糖的技术。[方法]采用静态吸附与动态试验相结合的方式,研究大孔树脂对不同聚合度麦芽聚糖吸附能力的差异。[结果]大孔树脂可吸附2倍柱体积麦芽糖浆中的多糖,且所吸附多糖无法被去离子水洗脱,而只能用95%乙醇洗脱。所用大孔树脂可有效吸附麦芽糖浆中的低聚糖,且其对麦芽低聚糖的吸附能力与低聚糖的聚合度呈正相关;同时,大孔树脂所吸附的麦芽低聚糖可以被去离子水洗脱。在50～150 mg/ml范围内,麦芽糖浆上样浓度越低,大孔树脂对低聚糖的分离效果越明显。[结论]利用大孔树脂分离制备麦芽低聚糖是可行的。

[Objective] The purpose was to introduce the preparation technique of malto-oligosaccharide using macroporous resin adsorptive process.[Method] By combining static adsorption and dynamic adsorption,the adsorbability difference of macroporous resin to maltosan of different polymerization degrees was studied.[Result] The polysaccharide in maltose syrup could be adsorbed by macroporous resin and its saturated adsorption amount was the oligosaccharide content in altose syrup of doubled column volume.The adsorbed polysaccharide could not be eluted with deionized water,but only be eluted with 95% ethanol.The used macroporous resin could adsorp oligosaccharide from maltose syrup effectively and its adsorbability on malto-oligosaccharide showed positive correlation with the polymerization degree of oligosaccharide.Simultaneously,the adsorped malto-oligosaccharide on macroporous could be eluted with deionized water.In the concn.range of 50~150 mg/ml,the lower the loading concn.of maltose syrup was,the more obvious the separation effect of macroporous resin on oligosaccharide was.[Conclusion] It was feasible to separating and preparing malto-oligosaccharide using macroporous resin.