急性髓性白血病患者herg mRNA的表达及临床意义

Expression of herg mRNA in patients with de novo acute myeloid leukemia and its clinical significance

目的:了解hergmRNA在急性髓性白血病(acutemyeloid leukemia,AML)患者中的表达情况,并初步分析其临床意义。方法:通过实时荧光定量PCR方法检测48例初诊AML患者和15例正常人外周血hergmRNA的表达情况,分析hergmRNA的表达水平与AML患者的临床与实验室特征以及患者的疗效和复发的关系。结果:48例初诊AML患者检测到38例(79.17%)外周血表达hergmRNA,初诊AML患者及正常人外周血hergmRNA表达水平的中位值分别为0.03(0.00～2.49)%和0.35(0.04～1.09)%,AML患者明显低于正常人(P=0.00),AML患者外周血hergmRNA的表达水平与其临床和实验室特征无明显相关性。不表达hergmRNA患者的完全缓解率(9/10,90%)高于表达的患者(31/38,81.58%),复发率前者低于后者,但差异无统计学意义(P=0.53,0.27)。结论:初诊AML患者外周血hergmRNA的表达水平明显低于正常人,尚未发现hergmRNA的表达情况与疾病预后的明确关系。

Objective To explore the expression level of herg mRNA in patients with de novo acute myeloid leukemia （AML） and to analyze its clinical significance. Methods Expression levels of berg mRNA in peripheral blood were detected by real-time quantitative PCR in 48 patients and 15 healthy subjects. The relation of berg mRNA level with the clinical and hematological characteristics and treatment outcomes （CR rate and relapse rate）wero analyzed. Results The expression of berg mRNA was detectable in 38 of 48 （79.17%） patients with AML. The median value of expression level was markedly lower in the patients than in the healthy controls （0.03% [0.00 ± 2.49]% vs. 0.35% [0.04 - 1.091%,P = 0.00）. The expression level of berg mRNA was not obviously related with the clinical and hematological characteristics. The rate of complete remission was higher in the berg mRNA-negative patients than in those with herg mRNA expression （90% vs. 81.58% ,P = 0.53）, and the relapse rate was lower （44.44% vs. 65.38%,P = 0.27）. Conclusions The patients with acute myeloid leukemia have a lower expression level of berg mRNA than the healthy people. No definite association of berg mRNA expression with the prognosis of the disease is found.