Lewis y抗原及α1,2-岩藻糖转移酶基因在卵巢癌细胞系中的表达

Expressions of Lewis y Antigen and α1,2-fucose Transferase Gene in Ovarian Cancer Cell Line

目的探讨卵巢癌细胞系中Lewisy抗原及α1,2-岩藻糖转移酶(α1,2-FT)基因FUT1表达变化对卵巢癌细胞增殖、耐药和转移的影响。方法采用免疫细胞化学法、免疫细胞荧光法测定α1,2-FT基因FUT1转染前后的人卵巢癌细胞系RMG-I和RMG-I-H、人卵巢癌高转移细胞系HO8910PM及亲本细胞系HO8910、人卵巢癌耐药细胞系COC1/DDP及亲本细胞系COC1中Lewisy抗原的表达。利用RT-PCR法及real-time PCR法检测上述6种细胞中FUT1基因表达的变化。结果经免疫化学方法和免疫细胞荧光法分析,在RMG-I-H、HO8910PM及COC1/DDP细胞系中Lewisy表达强度分别为(53.90±4.33),(37.31±0.19)和(28.52±1.45),与相应的亲本细胞系RMG-I(32.18±0.64)、HO8910(14.96±0.61)及COC1(19.26±0.83)比较均明显升高(P均<0.05)。与RMG-I相比,RMG-I-H中的FUT1基因表达上调3.07倍,与HO8910相比,HO8910PM中的FUT1基因表达上调2.13倍,与COC1相比,COC1/DDP中的α1,2-FT基因表达上调2.42倍(P均<0.05)。结论人卵巢癌细胞表面Lewisy抗原与卵巢癌细胞的增殖、耐药及转移等生物学行为密切相关。

Objective To study the expressions of Lewis y antigen and α1,2-fucose transferase（α1,2-fucosyltransferases,α1,2-FT） gene FUT1 in ovarian cancer cell lines and their influences on proliferation,drug resistance and metastasis of ovarian cancer cells.Methods The immuocytochemical method and immunocyto-fluorescence methold were used to detect the expression of Lewis y antigen in RMG-I-H and RMG-I,highly metastatic human ovarian cancer cell line HO8910PM and the parental cell line HO8910,drug-resistant human ovarian cancer cell line COC1/DDP and the parental cell line COC1,which were transfected by α1,2-FT gene（FUT1）.Real-time PCR and RT-PCR were used to determine the changes of α1,2-FT expression in these six cell lines.Results Results of the immunochemistry and immunofluorescence analysis showed that the expression intensities of Lewis y in RMG-I-H（53.90±4.33）,HO8910PM（37.31±0.19）,COC1/DDP（28.52±1.45）were higher than the corresponding parental cell lines RMG-I（32.18±0.64）,HO8910（14.96±0.61）,COC1（19.26±0.83）（P 0.05）.The mRNA expressions of α1,2-fucosyltransferases in RMG-I-H,HO8910 PM and COC1/DDP were higher than the corresponding cell lines RMG-I,HO8910 and COC1（P 0.05）.Conclusion Lewis y antigen on human ovarian cancer cell surface is closely related to the proliferation,drug resistance and metastasis of ovarian cancer cells.