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PCR-SSCP技术分析线粒体DNA多态性及其法医学应用 被引量：3

The Analysis of Mitochondrial DNA Polymorphism By PCR-SSCP and its Application in Forensic Medicine
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摘要 目的探讨SSCP法在线粒体DNA分析中的应用及其在法医实际检案中的意义。方法利用PCR方法直接扩增D-LOOP区的HVI16106-16297区域192bp片段作为目的片段,进行SSCP分型。检验150例随机个体、10例正常组织和5例甲醛固定石蜡包埋的组织。结果在150例随机个体中,SSCP检测发现9种单倍型,DP值为0.881。在10例正常组织中,各个组织的SSCP带型完全相同。5例包埋组织的SSCP分型与对照组织一致。结论 SSCP技术分析线粒体DNA有较高的鉴别能力,适用于陈旧降解检材的检验。 Objective To study the application of SSCP technology in the mitochondrial DNA analysis.Methods The highly variable region 16106-16297 in D-loop was amplified by PCR.150 unrelated Liaoning Han individuals,10 normal tissues and 5 formalin-fixed,paraffin-embedded tissues were investigated and analyzed by SSCP.Results In 150 unrelated Liaoning Han individuals,9 haplotypes were found by PCR-SSCP,DP=0.881.The 5 paraffin-embedded tissues displayed alleles different from those of corresponding normal tissue.Conclusion PCR-SSCP technology has a higher ability of mitochondrial DNA identification and is suitable for tests of degraded samples.
作者 张明阳 单海燕 徐冬冬 丁梅 王保捷 庞灏 周勤虎 冯春梅
机构地区 南通大学医学院法医学教研室 南通大学附属医院普外科 川北医学院法医系 中国医科大学法医学院法医血清学教研室
出处 《中国医科大学学报》 CAS CSCD 北大核心 2011年第4期334-336,共3页 Journal of China Medical University
关键词 法医物证学 多态性 PCR-SSCP技术 甲醛固定石蜡包埋组织 forensic physical evidence polymorphism PCR-SSCP technology formalin-fixed and paraffin-embedded tissue
分类号 R89 [医药卫生—法医学]
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参考文献8

  • 1Santos MC,Saito CP,Line SR. Extraction of genomic DNA from parafin-embedded tissue sections of human fetuses fixed and stored in fommlin for long periods [J]. Pathol Res Pract,2008,204 (9): 633-636.
  • 2Rivero ER,Neves AC,Silva-valenzu MG,et al. Simple salting-out method for DNA extraction from formalin-fixed,paraffin-embedded tissues [ J 1- Pathol Res Pract, 2006,202(7 ) : 523-529.
  • 3谭卓毅,丁梅,王保捷.福尔马林固定石蜡包埋组织中DNA提取[J].法医学杂志,2006,22(6):455-458. 被引量：32
  • 4McKinney MD,Moon SJ,Kulesh DA,et al.Deteetion of viral RNA from paraffin-embedded tissues after prolonged formalin fixation [ J ].J Clin Virol, 2009,44 ( 1 ) :39-42.
  • 5艾红伟,余纯应,赵贵森,黄代新,柯技,岳霞,杨庆恩.SSCP法mtDNA分型的法医学应用[J].法医学杂志,2006,22(2):117-119. 被引量：1
  • 6翟仙敦,尹慧,黄代新,杨庆恩.mtDNA异质性及其法医学应用[J].法律与医学杂志,2006,13(2):140-143. 被引量：2
  • 7Grzybowski T.Extremely high levels of human mitochondrial DNA heteroplasmy in single hair roots IJ].Eleetrophoresis,2000,21(3): 548-553.
  • 8Seo SB, Jang BS, Zhang A, et al.Alterations of length heteroplasmy in mitochondrial DNA under various amplification conditions [Jl- J Forensic Sci ; 2010,55 (3) : 719-722.

二级参考文献37

  • 1张金生 刘鸿瑞.两种提取石蜡包埋组织DNA方法的比较[J].诊断病理学杂志,2000,2:83-83.
  • 2Wilson MR,DiZinno JA,Polanskey D,et al.Validation of mitochondrial DNA sequencing for forensic casework analysis[J].Int J Legal Med,1995,108:68-74.
  • 3Orita M,Iwahana H,Kannazawa H,et al.Detection of polymorphisms of human DNA by gel electrophoresis as single-strand conformation polymorphism[J].Proc Nat Acad Sci USA,1989,86:2766-2770.
  • 4konstantin Gruzdov,peak explorer[EB/OL].Version 1.11,http://www.peak.km.ru.
  • 5Lutz S,Weisser HJ,Heizmann J,et al.A third hypervariable region in the human mitochondrial D-loop[J].Hum Genet,1997,101:384
  • 6Lutz S,Weisser H J,Heizmann J,et al.Mitochondrial heteroplasmy among maternally related individuals[J].Int J Legal Med,1999,113:155～161
  • 7Hauswirth WW,Laipis PJ.Mitochondrial DNA polymorphism in a maternal lineage of Holstein cows[J].Proc Natl Acad Sci U S A,1982,79(15):4686～4690
  • 8Ashley MV,Laipis PJ,Hauswirth WW.Rapid segregation of heteroplasmic bovine mitochondria[J].Nucleic Acids Res,1989,17:7325～7330
  • 9Bendall KE,Macaulay VA,Baker JR,et al.Heteroplasmic point mutations in the human mtDNA control region[J].Am J Hum Genet,1996,59 (6):1276～1287
  • 10Gill P,Ivanov PL,Klimton C,et al.Identification of the remains of the romanov family by DNA analysis[J].Nature Genet,1994,6(2):130～135

共引文献32

同被引文献18

  • 1张幼芳.浙江汉族群体线粒体DNA多态性调查[J].浙江警察学院学报,2007,19(3):105-106. 被引量：3
  • 2杨文超,张晓东.快速PCR研究进展[J].中国生物工程杂志,2007,27(4):99-103. 被引量：13
  • 3Verheij S,Harteveld J,Sijen T.A protocol for direct and rapid multiplex PCR amplification on forensically relevant samples[J] .Forensic Sci Int Genet,2012,6(2):167-175.
  • 4Wang DY,Chang CW,Hennessy LK.Rapid STR analysis of single source DNA samples in 2 h[J] .Forensic Sci Int Genet Suppl Set,2009,2(1):115-116.
  • 5Laurin N,Fregeau C.Optimization and validation of a fast amplification protocol for AmpFLSTR profiler plus for rapid forensic human identification[J] .Forensic Sci Int Genet,2012,6(1):47-57.
  • 6Vallone PM,Hill CR,Podini D,et al.Rapid amplification of commercial STR typing kits[J] .Forensic Sci Int Genet Suppl Ser,2009,2(1):111-112.
  • 7Kishore R,Reef WH,Anderson VJ,et al.Optimization of DNA extraction from low-yield and degraded samples using the BioRobot EZ1 and BioRobot M48[J] .J Forensic Sci,2006,51 (5):1055-1061.
  • 8Pavlov AR,Pavlova NV,Kozyavkin SA,et al.Recent developments in the optimization of thermostable DNA polymerases for efficient applications[J] .Trends Biotechnol,2004,22 (5):253-260.
  • 9Collins PJ,Hennessy LK,Leibelt CS,et al.Developmental validation of a single-tube amplification of the 13 CODIS STR loci,D2S1338,D19S433 and amelogenin:the AmpFLSTR Identifiler PCR Amplification Kit[J] .J Forensic Sci,2004,49 (6):1265-1277.
  • 10Vallone PM,Hill CR,Butler JM.Demonstration of rapid multiplex PCR amplification involving 16 genetic loci[J] .Forensic Sci Int Genet,2009,3 (1):42-45.

引证文献3

二级引证文献9

中国医科大学学报
2011年 第4期

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