飞秒激光处理对水稻种子发芽和苗期生长的影响

Effect of Femtosecond Laser Treatment on Rice Seed Germination and Seedling Growth

以水稻品种龙稻5号种子为试材,以波长800nm、光斑半径2mm、功率650mW、频率1 000Hz的飞秒激光分别辐照3、5、7、9、11和13s后,研究飞秒激光辐照处理对水稻种子发芽和苗期生长的影响。结果表明:飞秒激光辐照处理可以刺激水稻发芽和幼苗的生长,3～9s飞秒激光辐照处理,水稻发芽势和发芽率增高,而随着处理时间变长11～13s出现抑制发芽现象。苗期经处理后水稻植株比对照有不同程度增加现象,在11和13s处理株高变化较为明显。经辐照处理后幼苗叶绿素含量增加,POD酶活性增强,SOD酶活性减弱。

The rice seeds of Longdao No.5 were selected as experimental materials.The seeds of Longdao No.5 were exposed by femtosecond laser at the wavelength of 800 nm,spot radius of 2 mm,power 650 mW and frequency of 1 000 Hz for 3 s,5 s,7 s,9 s,11 s and 13 s,separately.The effect of femtosecond laser irradiation on rice seed germination and seedling growth were studied.The results showed that the germination and the seedling growth of rice were stimulated by exposing the rice seeds with femtosecond laser.3～9 s femtosecond laser irradiation increased the rice germinating viability and germination rate.The inhibition of sprouting happened with the 11～13 s femtosecond laser irradiation.The height of the rice plant was increased by exposing the rice seeds especially when the seeds were exposed for 11 s and 13 s.The chlorophyll content,the POD activity increased and the SOD activity decreased after the irradiation treatment.