白藜芦醇对慢性肝损伤模型小鼠的肝保护作用研究

Protective Effect of Resveratrol on Chronic Liver Injury Mice

目的:研究白藜芦醇对慢性肝损伤模型小鼠的肝保护作用及内毒素(Lps)刺激引起肝脏枯否细胞(KCs)分泌细胞因子的变化。方法:取小鼠36只随机均分为正常对照组(正常饮食)、模型组(高脂饮食)、药物组(高脂饮食,0.5%白藜芦醇10mL·kg-1)、阳性对照组(高脂饮食,联苯双酯150mg·kg-1),每隔1日灌胃给药1次,10周后每组抽取7只小鼠,腹主动脉采血测定血浆中丙氨酸氨基转移酶(ALT)、白细胞介素-6(IL-6)、IL-10、肿瘤坏死因子-α(TNF-α)水平;分离培养肝脏KCs,检测在Lps刺激下KCs上清液中IL-6、IL-10、TNF-α的水平。结果:与模型组比较,药物组和阳性对照组小鼠血浆中ALT、IL-6水平明显降低(P<0.05),IL-10、TNF-α无明显变化;小鼠肝脏KCs上清液中IL-6、TNF-α水平明显降低,IL-10水平明显升高(P<0.05或P<0.01)。结论:白藜芦醇可能通过降低KCs对促炎因子(IL-6、TNF-α)的表达,提高KCs对抑炎因子(IL-10)的表达,来实现其肝脏保护作用。

OBJECTIVE： To study the protective effect of resveratrol on chronic liver injury mice and the change of cytokines secreted by Kupffer cell（KCs） in response to endotoxin （Lps） stimulation. METHODS： 36 mice were randomly divided into normal control group （normal diet）, model group （high-fat diet）, drug group （high-fat diet, 0.5% resveratrol 10 mL.kg^-1）, positive control group （high-fat diet, bifendate 150 mg.kg^-1）. Each group was given medicine every other day. After 10 weeks, 7 mice of each group were sacrificed, blood sample was collected from abdominal artery for testing the levels of ALT, IL-6, IL-10, TNF-α. KCs was isolated and cultured to detect the levels of IL-6, IL-10, TNF-α in KCs supernatants after Lps stimulation. RESULTS： Compared with model group, the levels of ALT and IL-6 in drug group and positive group were decreased significantly （P〈0.05） while the levels of IL-10 and TNF-α had no significant changes. The levels of IL-6 and TNF-α in KCs supernatants decreased significantly while the level of IL-10 increased （P〈0.05 or P〈0.01）. CONCLUSION： Resveratrol might protect mice liver from chronic injury by decreasing the expression of IL-6 and TNF-α and increasing the expression of IL-10.