酶标仪校准方法探讨

Discussion of calibration method for ELISA analytical instruments

目的:建立一种酶标仪常规校准方法。方法:配制重铬酸钾溶液,用分光光度计450nm波长5mm比色杯比色结果与酶标仪主波长450nm参考波长630nm微孔板微孔内加样高度为5mm的比色结果进行比较,计算酶标仪的吸收度示值误差、示值稳定性、吸收度重复性、通道差异及相关性。结果:酶标仪吸收度示值误差、示值稳定性、吸收度重复性及通道差异均符合标准,且相关性良好,相关系数为0.999787。结论:该校准方法操作简单、结果可靠,可作为实验室常规校准酶标仪的方法。

Objective：To establish a conventional calibration method for enzyme micro-plate reader.Method：Prepared potassium bichromate solutions and compared the Absorbance determined by spectrophotometer and Optical Delnsity determined by enzyme micro-plate reader while Spectrophotometer Wavelength=450nm,Cuvette light path=5mm;Enzyme micro-plate reader Dominant wavelength=450nm,Reference wavelength=630nm,Height of porous microplate = 5mm.Calculated the indication error,stability,repeatability of Absorbance,differences of different porous microplate and correlations between spectrophotometer and enzyme micro-plate reader.Result：The indication error,stability,repeatability,differences of different porous microplate were all meeting the standard,and correlations was quite good,and the correlation coefficient was 0.999787.Conclusion：This method can be used as a conventional calibration method for enzyme micro-plate reader because of its simplicity of operator and reliable results.