含母牛分枝杆菌菌苗凝胶肺内应用的免疫效应研究

Study on immune response of Mycobacterium vaccae gel administered in lung

目的观察含母牛分枝杆菌菌苗（微卡）凝胶在肺部介入应用后产生的局部及全身免疫应答效应。方法60只日本长耳兔随机分成5组，每组12只。第1组为对照组，气管插管至末梢支气管注入生理盐水3ml；第2组为单纯凝胶组，肺部注入卡波姆凝胶3ml；第3、4、5组为微卡凝胶组，分别注入含3．75、7．50、9．37mg／L母牛分枝杆菌菌苗的卡波姆凝胶3ml。持续观察4周，期间检测首次注入后与处死解剖前外周血T细胞亚群及γ-干扰素含量。结果处死解剖前第4、5组外周血CD3^＋百分比[（55．84-9．7）％、（54．5±11．8）％]高于第1组[（43．1±3．0）％]（P值均〈0．05）；第4、5组外周血CD4^＋百分比[（44．1±5．8）％、（43．3±6．2）％]高于第1组[（33．5±3．1）％]（P值均〈0．05）；第3、4、5组外周血CD8^-百分比[（21．3±3．3）％、（21．1±4．5）％、（20．5±4．1）％]低于第1组[（28．2±1．7）％]（P值均〈0．05）。第4、5组检测微卡凝胶注入侧肺组织研磨混浆内γ-干扰素水平为阳性。结论以健康日本长耳兔为模型局部免疫刺激可以引起全身免疫应答反应。

Objective To observe local and systemic immunity effect of Mycobacterium vaccae gel administered in lung. Methods 60 Japanese long-eared rabbits were randomly divided into five groups. Group 1 was injected with 3 ml normal sodium. Group 2 was injected with 3 ml carbomer gel. Group 3,4, 5 were injected with 3 ml carbomer gel with 3.75,7.50,9.37 mg/L Mycobacterium vaccae. The peripheral blood T cell subsets and interferon-γwere tested. Results Before sacrificed ,CD3^＋ percents in group 4 and group 5 [（55.8± 9.7） % and （54.5± 11.8） %] were higher than that in group 1 [（43.1±3.0） %] （all P 〈0.05）,CD4^＋ percents in group 4 and group 5 [（44.1±5.8） % and （43.3±6.2） %] were higher than that ingroupl [（33.5±3.1）%] （all P 〈0.05）,CD8^＋ percents in group 3, 4, 5 [（21.3±3.3）%, （21.1±4.5）% and （20.5±4.1）%] were lower than that in group 1 [（28.2±1.7）%] （all P 〈0. 05）. Interferon-γ was positive in the lung tissues injected with carbomer gel with Mycobacterium vaccae in group 4 and group 5. Conclusions Local immune stimulation can cause systemic immune response in healthy Japanese long-eared rabbits.