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雄激素及其受体在大鼠多囊卵巢综合征发病机制中的相互作用 被引量：1

Interaction of androgen and its receptor in the pathogenesis of polycystic ovary syndrome in rats
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摘要 目的:探讨雄激素及其受体在多囊卵巢综合征发病机制中的相互作用。方法:通过建立来曲唑所致PCOS大鼠模型,其中实验组灌胃服用来曲唑1 mg.kg-1.d-1,溶于1%羧甲基纤维素中,实验对照组灌胃服用1%CMC,空白对照组不给以灌胃刺激,连续灌服21天。同时用放射免疫方法测定大鼠血清中雄激素水平,应用Western Blot方法观察AR在子宫内膜中的表达。结果:实验组血清T浓度显著高于实验对照组及空白对照组(P<0.05),实验组AR水平明显低于实验对照组及空白对照组(P<0.05)。结论:雄激素及其受体在子宫内膜中的表达及其相互作用与PCOS的发病密切相关。 Objective：To explore the interaction of androgen and its receptor in the pathogenesis of polycystic ovary syndrome（PCOS） in rats. Methods：The rat models of PCOS were established by letrozole,the rats in experimental group were treated by pouring letrozole（1 mg·kg^-1·d^-1 dissolved in 1% carboxymethylcellulose） into stomach for 21 days,the rats in experimental control group were treated by pouring 1% carboxymethylcellulose into stomach for 21 days,the rats in blank control group did not received any treatment.Radioimmunoassay was used to detect the serum level of androgen,Western Blot was used to observe the expression of androgen receptor in endometrium of rats. Results：The serum level of androgen in experimental group was significantly higher than those in experimental control group and blank control group（P〈0.05）,the serum level of androgen receptor in experimental group was significantly lower than those in experimental control group and blank control group（P〈0.05）. Conclusion：The expressions of androgen and its receptor and their interaction are related to the pathogenesis of PCOS closely
作者 胡颜霞 张展 贾莉婷
机构地区 河南省郑州市人民医院 郑州大学第三附属医院
出处 《中国妇幼保健》 CAS 北大核心 2011年第11期1694-1696,共3页 Maternal and Child Health Care of China
关键词 多囊卵巢综合征 动物模型 芳香化酶 雄激素受体 Polyeystic ovary syndrome Animal model Aromatase Androgen receptor
分类号 R711.75 [医药卫生—妇产科学]
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参考文献10

  • 1Brandsteffer AM, Pfaffl MW, Hocquette JF et al. Effects of muscle type,castration, age, and compensatory growth rate on androgen receptor mRNA expression in bovine skeletal muscle [J]. J Anita Sci, 2000, 78: 629.
  • 2胡颜霞,张展,贾莉婷.来曲唑诱导多囊卵巢综合征大鼠模型的研究[J].中国妇幼保健,2006,21(7):957-959. 被引量：34
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二级参考文献8

  • 1Kafali H,Iriadam M,Ozardl I et al.Letrozole-Induced Polycystic Ovaries in the Rat:A New Model for Cystic Ovarian Disease.Archives of Medecal Research,2004,35 (10):103.
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  • 5Primus EM,Yoshimura N,Kuhlmann B et al.Ar6matase Deficiency in a Female Who Is Compound Heterozygote for Two New Point Mutations in the P450arom Gene:Impact of Estrogens on Hypergonadotropic Hypogonadism,Multicystic Ovaries,and Bone Densitometry in Childhood.Clin Endocrinol Metab,1997,82 (6):1739.
  • 6Belgorosku A,Pepe C,Marino R et al.Hypothalamic-Pituitary-Ovaian Axis during Infancy,Early and Late Prepuberty in an Aromatase -Deficient Girl Who Is a Compound Heterocygote gor Two New Poing Mutations of the CYP19 Gene.Clin Endocrinol Metab,2003,88 (11):5127.
  • 7张晓薇,邝健全,曾爱群,刘慧姝.脱氢表雄酮诱导多囊卵巢综合征动物模型的研究[J].广州医学院学报,2000,28(3):14-18. 被引量：55
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中国妇幼保健
2011年 第11期

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