摘要
目的:通过一次性注射链脲佐菌素(STZ)制作大鼠糖尿病模型,观察大鼠品系、给药剂量对大鼠成模率、死亡率的影响。方法:Wistar、SD大鼠雄性各50只,随机分组为正常对照和剂量组(STZ1组:55mg/kg,2组:45 mg/kg,3组:35 mg/kg,4组:25 mg/kg),腹腔注射STZ,48小时后观察血糖,8周后计算死亡率,病理切片观察胰岛细胞。结果:Wistar、SD大鼠55mg/kg组全部成模,死亡率分别是100%、90%;45 mg/kg组全部成模,死亡率40%、28%;35 mg/kg组成模率为100%、30%,零死亡;25 mg/kg组成模率为70%、0%,零死亡。结论:35mg/kg剂量Wistar大鼠,成模率高,死亡率低,且结果优于SD大鼠。
Objective:To investigate the effects of streptozocin(STZ) dosage and the strains of rats on the incidence and mortality of diabetes mellitus animal model.Methods:50 Wistar male rats and 50 SD male rats were randomly divided into normal control group and dose group;dose group were injected intraperitoneally with different dose of STZ(group 1,STZ 55mg/kg,group 2,STZ 45 mg/kg,group 3,STZ 35 mg/kg,group 4,STZ 25 mg/kg).Then,the blood glucose was measured after 48 hours,calculating mortality and to observe pathological change of islet cells after 8 weeks.Results:1 The incidence of diabetes mellitus:was 100% in both group 1 and group 2,of both Wistar rats and SD rats.But in group 3,it was 100% in Wistar rats,but only 30% in SD rats;in group 4,it was 70% in Wistar rats,0% in SD rats.2.The mortality rate:in group 1 was 100% in wistar rats,90% in SD rats;in group 2,it was 40% in wistar rats,28% in SD rats,and it was 0% in either wistar rats or SD rats in both group 3 and group 4.Conclusion:The incidence is higher and the mortality rate is lower for wistar rats with moderate dose of STZ(35mg/kg).
出处
《泸州医学院学报》
2011年第2期156-158,共3页
Journal of Luzhou Medical College
关键词
链脲佐菌素
糖尿病
成模
大鼠
Streptozotocin
Diabetes mellitus
DM model establishment
Rat