大鼠品系及药物剂量对糖尿病大鼠模型的影响

EFFECTS OF STREPTOZOCIN DOSAGE AND THE STRAINS OF RATS ON ESTABLISHING DIABETES MELLITUS ANIMAL MODEL

目的:通过一次性注射链脲佐菌素(STZ)制作大鼠糖尿病模型,观察大鼠品系、给药剂量对大鼠成模率、死亡率的影响。方法:Wistar、SD大鼠雄性各50只,随机分组为正常对照和剂量组(STZ1组:55mg/kg,2组:45 mg/kg,3组:35 mg/kg,4组:25 mg/kg),腹腔注射STZ,48小时后观察血糖,8周后计算死亡率,病理切片观察胰岛细胞。结果:Wistar、SD大鼠55mg/kg组全部成模,死亡率分别是100%、90%;45 mg/kg组全部成模,死亡率40%、28%;35 mg/kg组成模率为100%、30%,零死亡;25 mg/kg组成模率为70%、0%,零死亡。结论:35mg/kg剂量Wistar大鼠,成模率高,死亡率低,且结果优于SD大鼠。

Objective：To investigate the effects of streptozocin（STZ） dosage and the strains of rats on the incidence and mortality of diabetes mellitus animal model.Methods：50 Wistar male rats and 50 SD male rats were randomly divided into normal control group and dose group;dose group were injected intraperitoneally with different dose of STZ（group 1,STZ 55mg/kg,group 2,STZ 45 mg/kg,group 3,STZ 35 mg/kg,group 4,STZ 25 mg/kg）.Then,the blood glucose was measured after 48 hours,calculating mortality and to observe pathological change of islet cells after 8 weeks.Results：1 The incidence of diabetes mellitus：was 100% in both group 1 and group 2,of both Wistar rats and SD rats.But in group 3,it was 100% in Wistar rats,but only 30% in SD rats;in group 4,it was 70% in Wistar rats,0% in SD rats.2.The mortality rate：in group 1 was 100% in wistar rats,90% in SD rats;in group 2,it was 40% in wistar rats,28% in SD rats,and it was 0% in either wistar rats or SD rats in both group 3 and group 4.Conclusion：The incidence is higher and the mortality rate is lower for wistar rats with moderate dose of STZ（35mg/kg）.