鼻咽癌患者EBV感染与血管生成关系研究

Correlation between Epstern-Barr virus andangiogenesis of patients with nasopharyngeal carcinoma

目的探讨鼻咽癌患者EBV感染与血管生成的关系。方法采用PCR技术检测54例鼻咽癌患者EBV DNA,采用双抗夹心ELISA方法检测该54例鼻咽癌患者血清VEGF水平,并与40例健康人血清VEGF水平进行比较。结果 54例鼻咽癌患者血清VEGF水平(143.86±19.49)pg/mL显著高于40例健康人血清VEGF水平(51.23±12.66)pg/mL,(P<0.01);24例鼻咽癌EBV阳性患者血清VEGF水平(195.44±19.69)pg/mL,明显高于30例鼻咽癌EBV阴性患者血清VEGF水平(154.58±15.23)pg/mL,(P<0.01)。结论鼻咽癌患者血清VEGF水平变化有可能作为一种新的肿瘤标志物对鼻咽癌病情进展进行动态观察,为临床治疗提供客观指标。

Objective To explore correlation between Epstern-Barr virus and angiogenesis of patients with nasopharyngeal carcinoma. Methods Epstern - Barr virus DNA of 54 patients with nasopharyngeal carcinoma was measured by PCR and their serum vascular endothelial growth factor （serum VEGF） level was also detected by ELISA, and these data was compared and analyzed with serum vascular endothelial growth factor level of 40 health control. Results Serum vascular endothelial growth factor level of 54 patients with nasopharyngeal carcinoma （143.86 ±19.49） pg/ mL was very higher than that of 40 health control （51.23 ± 12.66） pg/mL, （P 〈 0.01 ） ; Serum vascular endothelial growth factor level of 24 EBV positive patients with nasopharyngeal carcinoma （195.44 ±19.69） pg/mL was remarkable higher than that of 30 EBV negative patients with nasopharyngeal carcinoma （154.58±15.23 pg/mL）, （P〈0.01）. Conclusion Serum vascular endothelial growth factor level changed in patients with nasopharyngeal carcinoma may be as a new tumor mark for determining disease advancing about nasopharyngeal carcinoma.