埃兹蛋白、转移相关黏附分子44V6、E-钙黏附素在人结肠癌组织中的表达及其意义

Expression of Ezrin,CD44V6 and E-cadherin in Tissues of Colon Carcinoma and Its Significance

目的探讨埃兹蛋白(Ezrin蛋白)、转移相关黏附分子44V6(CD44V6)、E-钙黏附素在人结肠癌组织中的表达及意义。方法应用免疫组化S-P法检测Ezrin、CD44V6、E-钙黏附素在78例结肠癌组织、30例结肠腺瘤组织、30例正常结肠黏膜中的表达,分析三者与临床病理特征的关系及相关性。结果结肠癌组织中Ezrin、CD44V6蛋白表达阳性率显著高于结肠腺瘤组织及正常结肠组织(P<0.01),而有淋巴转移者显著高于无淋巴转移者(P<0.05);E-钙黏附素蛋白表达阳性率显著低于结肠腺瘤及正常结肠黏膜组织(P<0.01),无淋巴转移组明显高于有淋巴转移组(P<0.01)。结肠癌组织中Ezrin、CD44V6蛋白表达水平与肿瘤进展及转移呈正相关(P<0.05),而E-钙黏附素蛋白表达水平与肿瘤进展及转移呈负相关(P<0.05)。结论 Ezrin、CD44V6、E-钙黏附素可能与结肠癌的侵袭转移相关,三者联合检验能更准确地判断结肠癌的转移情况。

Objective To investigate the expression of Ezrin,CD44V6 and E-cadherin in tissues of colon carcinoma and its significance.Methods Immunohistochemistry（IHC） method（SP method）was used to examine the expression of Ezrin,CD44V6 and E-cadherin in 78 cases colon carcinoma,30 cases colorectal adenomas and 30 cases normal colonic mucosa.Their relationships with clinicopathological features were analyzed.Results The positive expression rate of Ezrin and CD44V6 in colon cancer tissues was significantly higher than that in colorectal adenomas and normal colonic mucosa tissues（P0.01）;while positive expression rate in tumors with lymph node metastasis was significantly higher than those in tumors without lymph node metastasis in colon carcinoma（P0.05）;The positive expression rate of E-cadherin in colon cancer tissue was significantly lower than that in colorectal adenomas and normal colonic mucosa tissue（P0.01）,and positive expression rate in tumors without lymph node metastasis was significantly higher than those in tumors with lymph node metastasis in colon carcinoma（P0.01）.The expression levels of Ezrin and CD44V6 were positively correlated with lymph node metastasis and clinical stage（P0.05）,the expression level of E-cadherin was negatively correlated with lymph node metastasis and clinical stage（P0.05）.Conclusion The expression of Ezrin,CD44V6 and E-cadherin is related with the attack and metastasis of colon carcinoma.Combined testing of Ezrin,CD44V6 and E-cadherin can judge more accurately the metastasis of colon carcinoma.