中国美利奴绵羊55L9 BAC克隆基因的筛选与序列的初步分析

Screening and sequencing of candidate genes in Chinese merino sheep 55L9 BAC clone

利用绵羊55L9 BAC(细菌人造染色体)克隆制备探针,采用噬菌斑原位杂交方法,从中国美利奴细毛羊cDNA文库中筛选与免疫相关的基因,并对筛选得到的候选基因进行测序及软件分析。初步筛出12个候选阳性克隆。经过测序和比对,推测有8个独立的序列可能与绵羊的免疫及疾病相关,其中2个基因可以在已提交的BAC序列中定位。

Sheep 55L9 BAC（bacterial artificial chromosomes） clones was used to prepare radioactive probes.The probes were used to screen the immune-related gene from Chinese merino cDNA library through phage in situ hybridization.The isolated candidate positive cDNA clones were sequenced and analyzed.Twelve candidate positive clones were screenedout,of which 8 separate sequences obtained by sequencing and blasting may be immune-related genes of sheep.Two genes in the 8 separate sequences were located on the 55L9 BAC.