蛋白微球机械剪切制备方法的研究

Study on mechanical shearing process for preparing protein microspheres

目的建立制备蛋白微球的机械剪切法。方法在不同工作条件下制备蛋白微球，通过检测微球大小、稳定性及其他质量指标来确定最佳工作条件。结果当将起始制备温度设定为65℃、转定子转速调至15000r／min时，机械剪切法可制备最稳定、质量最佳的蛋白微球，且可获得最高蛋白微球产量。结论机械剪切法可用于蛋白微球的规模化生产。

Objective To establish mechanical shearing process for preparing protein microspheres. Methods The protein microspheres were prepared by mechanical shearing process under various preparation conditions. The optimal preparation conditions were identified by detecting size, stability and other quality indexes of protein microspheres. Results When the initial preparation temperature was set up at 65 ~C and the rotating speed of rotor and stator was modulated to 15 000 r/min, the protein microspheres with the higest stability and quality could be prepared by mechanical shearing process, and the highest yields of protein microspheres were obtained. Conclusion Mechanical shearing process can be applied to preparing protein microspheres on a large scale.