EBV相关性IM患儿外周血淋巴细胞亚群特点

Peripheral blood lymphocytic subsets of children with EBV-associated IM

目的研究EB病毒感染传染性单核细胞增多症（IM）患儿不同时期外周血细胞免疫状况。方法分别在36例传染性单核细胞增多症患儿病程的急性期和恢复期采集外周血，通过流式细胞仪检测CD3＋、CD4＋、CD8＋及NK细胞的表达。同时选取34例正常同龄对照组儿童比较。结果传染性单核细胞增多症患儿急性期CD3＋（64．79±12．10％）CD8＋（40．21±16．13％）明显增高，CD4（29．01±11．83％）CD4＋／CD8＋（0．90±0．65）明显降低，与对照组及恢复期有明显差异，而NK（15．10±8．73）与对照组及恢复期无明显差异。对照组与传染性单核细胞增多症恢复期CD3＋、CD4＋、CD8＋、CD4／CD8及NK比较无明显差异。结论传染性单核细胞增多症患儿在疾病急性期外周血象细胞免疫较恢复期及正常患儿有明显改变。至病程1个基本恢复正常水平，NK细胞无明显变化。

Objective To investigate cellular immunity in peripheral blood of children with infectious mononucleosis （IM） caused by Epstein-Barr Virus（EBV） in different clinical stages. Methods The numbers of CD3^＋ , CD4^＋ , CD8^＋ , CD4＋/CD8^＋ and NK cells in peripheral blood of 36 children with IM at acute and restoration stages and 34 healthy children in same ages as controls were detected respectively by flow cytometry and compared. Results （1） The numbers of CD3 ^＋ （64.79 ±12.10% ） and CD8 ^＋ （40. 21± 16. 13% ） in peripheral blood of IM in the acute stage were significantly increased, which were significantly higher than those in both the restoration stage and the control group, while the numbers of CD4 （29.01 ± 11.83 % ）and CD4 ^＋/CD8 ^＋ （0.90 ± 0.65 ）were significantly decreased. But in the number of NK cells （ 15.10± 8.73 ） in peripheral blood there was no significant difference between IM children in the acute stage, IM children in the restoration stage and the controls; （2） In the numbers of CD3 ^＋ , CD4 ^＋ , CD8 ^＋ , CD4/CD8 and NK cells in peripheral blood, there were no significant differences between the controls and those IM children in the restoration stage. Conclusion The numbers of CD3 ^＋ and CD8 ^＋ cells in peripheral blood of IM children are significantly increased in the acute stage than those children with IM in the restoration group and the controls, while the numbers of CD4 ^＋ and CD4/CD8 were markedly decreased in the acute stage. At one onth of the course of the disease, the numbers of CD3 ^＋ , CD4 ^＋ , CD8 ^＋ and CD4/CD8 in peripheral blood returned to normal. But number of NK cells in the peripheral blood doesn＇ t change significantly.