植物乳杆菌B_(28)产细菌素的发酵条件研究

Optimization of bacteriocin production by Lactobacillus plantarum B_(28)

从来自保加利亚传统燕麦饮料中分离得到的植物乳杆菌B28出发,以腊样芽胞杆菌Baeillus cereus为指示菌,研究了植物乳杆菌B28生长特性以及产生细菌素的最佳时期;不同氮源、碳源和磷酸盐对细菌素抑菌活性性影响以及不同浓度硫酸铵溶液对细菌素的盐析效果。结果表明,植物乳杆菌B28在37℃条件下培养,14~16 h,生长进入稳定期;产生细菌素最佳发酵时间为24 h;70%的硫酸酸铵是适植物乳杆菌细菌素的盐析质量分数为。与对照培养基对比,植物乳杆菌B28的生长情况是酵母提取物〉大豆蛋白〉胰蛋白胨、蛋白胨、肉膏;乳糖〉D-果糖、蔗糖、D-麦芽糖〉D-木糖;磷酸盐对植物乳杆菌B28的生长影响不大。并得到了产生细菌素的最佳培养基配方。

Lactobacillus plantarum B28,which is a strain isolated from traditional Bulgarian oat beverage,produces a bacteriocin which is inhibitory to Baeillus cereus.The growth characteristic of Lactobacillus plantarum B28 and the optimal stage of producing bacteriocin were studied.The different cultures and different concentrations of ammonium sulphate effect on bacteriocin activity was observed.The growth cycle of B28 was studied at 37 ℃ which indicated its stationary phase starting from the 14 th hour to the 16th hour.The bacteriostatic activity of plantaricin,produced by the strain has been improving constantly.It reached the peak value at 24 h.L.Plantarum grew better in media in presence of yeast extract,soybean protein,lactose,D-fructose,sucrose,or D-maltose.D-xylos was not suitalbe for L.Plantarum.There was no considerable effect when it grew in KH2PO4 and NaH2PO4.The optimized medium was achieved.