光照调整对于牛乳中褪黑素质量浓度的影响及其检测

Influence of light rhythm on melatonin in milk and detection

通过调整奶牛的光照周期使牛乳中富含褪黑素成分,并通过液相色谱技术对于富含褪黑素的原料乳进行检测,结果发现褪黑素质量浓度有显著提高。色谱条件:色谱柱Zorbax eclipse XDB C18柱;流动相为甲醇∶水=60∶40,流速为1 mL/min,荧光检测器Ex=286 nm,Em=345 nm。

In order to get the melatonin rich milk,the method by adjusting the light rhythm of cows was used.And a method for determination of melatonin in milk and dairy products by high-performance liquid chromatography（HPLC） was used.And then separated on Zorbax eclipse XDB C18 column with a mixed solution of methanol/water 60/40 as mobile phase,and detected at Ex=286 nm,Em=345 nm with a Fluorescence detector.