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金黄地鼠颊癌差异表达基因谱的构建及相关生物信息学分析

The establishment of golden hamster cheek mucosa carcinoma related gene differential expression profile and bioinformatics analysis
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摘要 目的:构建二甲基苯并蒽(9,10-Dimethylen-1,2Benzanthracene,DMBA)诱导金黄地鼠颊囊黏膜癌变过程中基因差异表达谱,用相关生物信息学分析方法筛选在癌变过程中起重要作用的基因和信号通路。方法:用DMBA诱导建立金黄地鼠颊鳞癌模型,以Ratio≥2和Ratio≤0.5为阈值,用Agilent全基因表达谱芯片检测金黄地鼠颊囊黏膜癌变的差异表达基因,并对这些差异基因进行(Gene ontology,GO)功能分类和Pathway通路分析。结果:金黄地鼠正常颊黏膜和癌变组织之间共有1 943条差异表达基因,其中上调基因787条,下调基因1 156条;GO分析差异表达基因涉及结构分子相关基因群、转录调控相关基因群和代谢相关基因群等10类;Pathway分析在颊黏膜癌变中共有27条通路发生显著性异常改变,有136条差异基因在以上27条异常通路上发生富集,其中Ppp3r2、Actn1、Src等13条基因均分别富集在3条以上异常改变的通路上。结论:金黄地鼠颊癌的发生共有1 943条基因发生差异表达和27条通路发生异常改变,其中Ppp3r2、Actn1、Src等13条基因是导致细胞通路改变而最终发生癌变的重要致病基因,针对以上基因和通路的深入研究,将有助于揭示口腔黏膜癌变的分子机制,并为基因治疗提供新的靶点。 Objective:To construct differential gene expression profile in the DMBA(9,10-Dimethylen-1,2Benzanthracene,DMBA) induced hamster cheek pouch mucosa carcinogenesis process,using bioinformatics analysis methods in screening the genes and signaling pathways which play an important role in carcinogenesis.Methods:The DMBA-induced hamster buccal squamous cell carcinoma model was established;screening criteria of Ratio≥2 and Ratio≤0.5 were used;Agilent whole gene expression profiling microarray was used to detect the differentially expressed genes in hamster cheek pouch mucosa carcinogenesis,and then Gene Ontology for biological function and Pathway analysis were performed with those important virulent genes.Results:A total of 1 943 differentially expressed genes between normal hamster buccal mucosa and cancerous tissue were detected,of which 787 up-regulated and 1 156 down-regulated;GO analysis of differentially expressed genes involved structural elements,transcription regulation,metabolism and so on;pathway analysis found that 27 channels had significant changes,involving a total of 136 different genes.Among them,13 genes,including Ppp3r2,Actn1 and Src involved three or more pathways.Conclusion:During the occurrence of the golden hamster cheek cancer,a total of 1 943 genes were differentially expressed and 27 pathways abnormally changed,among which 13 genes including Ppp3r2,Actn1 and Src lead to changes in cellular pathways and eventually become an important cause of cancer.An in-depth study of those important genes will reveal the molecular mechanism of carcinogenesis of oral mucosa and provide an effective method of targeted therapy.
出处 《重庆医科大学学报》 CAS CSCD 北大核心 2010年第12期1782-1786,共5页 Journal of Chongqing Medical University
基金 重庆市卫生局科研项目(编号:03-2-073)
关键词 金黄地鼠 颊黏膜 癌变 全基因表达谱 生物信息学 Golden hamster Cheek pouch mucosa Carcinogenesis Entire gene expression profile Bio-informatics
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