酒精联合内毒素诱导慢性胰腺炎模型的建立

Establishment of models of chronic pancreatitis with ethanol and endotoxin

目的:用酒精联合内毒素建立一个新型的慢性胰腺炎(Chronic pancreatitis,CP)的大鼠模型,为研究CP的发病机制提供合适的动物模型。方法:18只1月龄雄性SD大鼠随机分为对照组、酒精组和模型组,每组各6只。饲以25%酒精,12周后反复腹腔注射脂多糖(Lipopolysaccharides,LPS),建立CP的动物模型。在光镜下观察胰腺组织显微结构变化,用Masson染色法观察胰腺组织胶原的改变,并测定胰腺组织匀浆中的淀粉酶和脂肪酶及血糖浓度。结果:模型组胰腺小叶内可见炎症细胞浸润,胶原纤维较对照组和酒精组明显增多,胰腺组织内淀粉酶和脂肪酶降低(P<0.05)。结论:在长期摄入乙醇的基础上反复腹腔注射LPS可造成CP发生,本模型可以用于CP的研究。

Objective：To investigate the pathogenesis of chronic pancreatitis（CP） by establishing a new pancreatitis model in rats suitable for investigation of the processes and mechanisms leading to pancreatic fibrosis.The model was established with ethanol and lipopolysaccharides（LPS,endotoxin）.Methods：Eighteen one-month old male SD rats were randomly divided into control group,alcohol group,and model group.After fed with 25% alcohol for twelve weeks,the rats in model group were injected with lipopolysaccharides（LPS）.Pancreatic morphology was conducted with haematoxylin-eosin stain,collagen deposition was determined by Masson stain,and the amylase and lipase in pancreatic tissue homogenate were evaluated by colorimetry.Results：Compared with control group,the acinar cells with vacuolization,inflammatory cell infiltration and collagen increasing were observed in the model group.Lipase and amylase were significantly decreased in the model group（P0.05）.Conclusion：Combined with ethanol,LPS can induce chronic pancreatitis.The rat model may be useful for studying the mechanism of chronic pancreatitis.