高压氧对急性一氧化碳中毒大鼠血清蛋白脂质蛋白质抗原、抗体的影响

Effects of hyperbaric oxygon on the content of PLP, PLP-antigen and antibody in serum in rats with acute carbon monoxide poisoning

目的 观察急性一氧化碳中毒（acute carbon monoxide poioning,ACOP）大鼠血清中蛋白脂质蛋白质（proteolipid protein,PLP）抗原、抗体的变化,探讨免疫机制在ACOP中的作用以及高压氧（HBO）治疗可能的作用机制.方法 202只大鼠按随机数字表法分为3组：染毒组（CO组）,高压氧组（HBO组）,常压氧组（NBO组）.采用静态一氧化碳吸入法建立ACOP大鼠模型.染毒前及染毒后即刻、100 min和3、7、10、20 d取腹主动脉血,用ELISA法测定血清PLP抗原、抗体的含量.结果 染毒后即刻CO组、HBO组和NBO组PLP抗原及抗体均较染毒前升高（P＜0.05）.HBO组100 min时PLP抗原低于CO组（P＜0.05）;3、7 d时PLP抗体低于CO组（P＜0.05）;3、10 d时HBO组PLP抗体低于NBO组（P＜0.05）;7、20 d时NBO组PLP抗体低于CO组（P＜0.05）.结论 ACOP后大鼠存在自身免疫反应及髓鞘损伤,及时、规律的HBO治疗可减轻髓鞘损伤.

Objective To observe effects of hyperbaric oxygen on the changes in proteolipid protein （PLP）, PLP antigen and antibody in serum in rats with acute carbon monoxide poisoning（ACOP） and to explore the role of the immune system in ACOP and also the mechanism that might be involved in. Methods Two hundred and two rats were randomly divided into 3 groups： the carbon monoxide （CO） group, the HBO group and the norrnobaric oxygen （NBO） group. The rat model of ACOP was established with static inhalation of CO. Abdominal aorta blood was collected before poisoning, the moment immediately after poisoning, 100 min, 3rd, 7th, 10th and 20th days after poisoning. Then, levels of PLP antigen and PLP antibody were measured with ELISA. Results For the CO group measured immediately after poisoning in the HBO group and NBO group, the levels of PLP antigen and antibody elevated, when they were compared with those before poisoning（P 〈0.05）. The level of PLP antigen at 100 min in the HBO group was lower than that of the CO group （P 〈 0.05 ）. The levels of PLP antibody in the HBO group were lower than those of the CO group on 3rd and 7th days（ P 〈 0. 05 ）. The levels of PLP antibody in the HBO group were lower than those of the NBO group on 3rd and 10th days（P 〈0.05）; The levels of PLP antibody in the NBO group were lower than those in the CO group on 7th and 20th days （ P 〈 0.05 ）. Conclusions Following acute carbon monoxide poisoning, levels of PLP antigen and PLP antibody increased in serum, which initiated impairment of myelin and an adaptive immunological response. Regular HBO and NBO treatment could alleviate lesion to the myelin following CO poisoning, and HBO treatment was more effective than NBO treatment.