Tiam1和Rac1在食管鳞癌组织中蛋白表达的相关性及临床病理意义

Clinicopathological significance of Tiam1 and Rac1 protein expression in esophageal squamous cell carcinoma

目的:探讨T淋巴瘤侵袭转移诱导因子1(Tiam1)及Rac GTP酶激活蛋白1(Rac1)的表达与食管鳞癌发生发展、浸润转移的关系.方法:62例食管癌手术切除标本于2006-02-26/03-16取自食管癌高发区河南省安阳市肿瘤医院.应用免疫组织化学SP法检测62例食管鳞癌组织、20例癌旁不典型增生组织及20例正常食管黏膜组织中Tiam1及Rac1蛋白表达.结果:食管鳞癌组织中Tiam1蛋白表达与癌的组织学分级、浸润深度、淋巴结转移及TNM分期均密切相关(χ2=8.779,7.680,4.502,4.987;均P<0.05);在食管鳞癌癌变过程中Tiam1蛋白表达在正常黏膜组织、癌旁不典型增生组织及癌组织中的表达率依次增高,分别为5.0%(3/20)、55.0%(11/20)、72.6%(45/62),组间比较有明显差异(χ2=20.643,P<0.05);Rac1蛋白表达也与癌的组织学分级、浸润深度、淋巴结转移及TNM分期均密切相关(χ2=8.652,6.884,8.276,6.371;均P<0.05);Rac1蛋白在正常黏膜组织、癌旁不典型增生组织及癌组织中的表达率依次增高,分别为25.0%(5/20)、70.0%(14/20)、70.0%(44/62),组间比较有明显差异(χ2=14.245,P<0.05).Tiam1及Rac1的表达呈正相关关系(γp=0.642,P<0.05).结论:Tiam1及Rac1可作为食管鳞癌早期诊断和判断预后的辅助指标.

AIM：To detect the expression of T-cell lymphoma invasion and metastasis factor1（Tiam1） and Rac GTPase activating protein 1（Rac1） proteins in esophageal squamous cell carcinoma（ESCC） and to analyze their correlation with the development,progression,invasion and metastasis of ESCC.METHODS：The expression of TIAM1 and Rac1 proteins was detected by immunohistochemistry in 62 ESCC specimens,20 tumor-adjacent atypical hyperplastic epithelial specimens,and 20 normal esophageal epithelial specimens.RESULTS：The expression of Tiam1 protein was closely correlated with tumor grade,infiltration,lymph node metastasis,and TNM grade in ESCC（χ2 = 8.779,7.680,4.502 and 4.987,all P 〈0.05）.There is a significant difference in the positive rate of Tiam1 protein expression among normal esophageal epithelium,tumor-adjacent atypical hyperplastic epithelium and carcinoma [15.0%（3/20）,55.0%（11/20） and 72.6%（45/62）,respectively;χ2 = 20.643,P 〈0.05].The expression of Rac1 protein was closely correlated with tumor grade,infiltration,lymph node metastasis,and TNM grade in ESCC（χ2 = 8.652,6.884,8.276 and 6.371,all P〈 0.05）.There is a signif icant difference in the positive rate of Rac1 protein expression among normal esophageal epithelium,adjacent atypical hyperplastic epithelium and carcinoma [25.0%（5/20）,70.0%（14/20） and 70.0%（44/62）,respectively;χ2 = 57.621,P 〈0.01].There is a positive correlation between the expression of Tiam1 and Rac1 proteins（γp = 0.642,P 〈0.05）.CONCLUSION：Combined detection of Tiam1 and Rac1 expression can be used for early diagnosis and prognostic evaluation of ESCC.