靶向阻断COX-2信号通路对喉癌细胞生长的影响

Effects of target blocking COX-2 on the growth of laryngeal cancer

目的观察赛来昔布靶向阻断COX-2信号通路对喉癌细胞生长的影响。方法用不同浓度的赛来昔布处理喉癌细胞Hep-2,通过MTT法检测赛来昔布对喉癌细胞的生长抑制率的影响。用浓度为15μmol/L的赛来昔布处理Hep-2细胞,通过流式细胞学检测药物处理前后细胞周期、凋亡率的差异,运用Western blot检测药物处理前后相关蛋白EGFR和COX-2表达的差异。结果赛来昔布处理后的Hep-2细胞与药物浓度成比例地出现生长抑制率增高;赛来昔布干预组细胞G1期比例大于对照组(P<0.01),凋亡率明显升高(P<0.05);与对照组相比,赛来昔布干预组出现明显的p-EGFR,COX-2表达降低(P<0.05)。结论赛来昔布能抑制喉癌细胞株Hep-2生长,增加Hep-2细胞G1期阻滞,降低肿瘤细胞增殖能力和诱导细胞凋亡;赛来昔布可抑制喉癌细胞COX-2的表达和EGFR的磷酸化。提示COX-2抑制剂可能作为喉癌靶向治疗的新方法。

Objective To explore the effect of COX-2 inhibitor（Celecoxib） on laryngeal cancer cell lines Hep-2.Methods Laryngeal cancer cell lines Hep-2 was treated with Celecoxib at various concentrations.Cell growth inhibition rate was measured by MTT.After Hep-2 cells were treated with Celecoxib at the concentration of 15 μmol/L,the cell cycle and apoptosis of the cells were measured by flow cytometric analysis.The expression of relative proteins was also examined using western blot.Results ① The cell growth inhibition rate of Celecoxib-treated group was much higher than that of the control,and the inhibition was concentration-dependent in a certain range.② The G1 ratio and apoptotic rate of Celecoxib-treated group were significantly higher than those of the control group respectively（P0.05）.③ Compared with the control group,the expression of p-EGFR and COX-2 in Celecoxib-treated group decreased significantly（P0.05）.Conclusion Celecoxib can inhibit the growth and induce apoptosis of Hep-2 cell lines via significant induction of Hep-2 cells G1.Meanwhile,Celecoxib can reduce phosphorylation of EGFR and expression of COX-2.Therefore,Celecoxib,as a COX-2 inhibitor,may be a new agent for targeting therapy of laryngeal cancer.