摘要
目的寻找并鉴定在膀胱癌及癌旁组织中差异表达的微RNA(miRNA)。方法结合miRNA芯片技术寻找膀胱癌及癌旁组织中差异表达的miRNA,并通过荧光定量PCR进行验证。结果在癌与癌旁组织中共检测到115条存在显著性差异的miRNA,其中膀胱癌组织中下调42条,上调73条。结论膀胱癌组织与癌旁组织中miRNA表达谱存在较大差异,miRNA与膀胱癌的发生发展存在一定的相关性。
Objective To identify the differentially expressed microRNA（miRNA） in bladder cancer.Methods The miRNA was detected by miRNA array and examined by qRT-PCR.Results A total of 115 differentially expressed miRNAs were detected by miRNA array in clinical bladder cancer and paracancer tissues,42 of which were low-expressed while 73 of them were highly-expressed in bladder cancer tissues.Conclusion The miRNA expression profile is different in bladder cancer and paracancer tissues,suggesting that miRNA might be involved in the initiation and development of bladder cancer.
出处
《军事医学科学院院刊》
CSCD
北大核心
2010年第6期540-542,共3页
Bulletin of the Academy of Military Medical Sciences
基金
国家重点基础研究发展计划(973计划)(2005CB724600)
国家自然科学基金(30600110
30971630)
国家高技术研究发展计划(863计划)(2007AA021004)
关键词
膀胱肿瘤
微RNA
聚合酶链反应
基因
肿瘤抑制
urinary bladder neoplasms
microRNA
polymerase chain reaction
genes
tumor suppressor
