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甘薯提取物有效成分鉴定及其对破骨细胞形成分化的影响 被引量：1

The identification of the effective component in sweet potato extracts and its effect on the formation and differentiation of osteoclast
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摘要 目的对甘薯提取物有效化学成份进行鉴定,并观察各有效成分对破骨细胞形成分化的影响。方法采用高效液相色谱对甘薯提取物有效成份进行鉴定,同时采用3种不同细胞培养系,观察甘薯提取物4种有效成分对破骨细胞形成分化的影响。结果甘薯提取物有效成分包括咖啡酸、绿原酸、异构绿原酸和奎宁酸。在4种有效成分中,咖啡酸对破骨细胞形成分化的影响最大,直接抑制破骨细胞的形成和分化,而奎宁酸未显示出有效的抑制作用。结论甘薯提取物4种有效成分对破骨细胞形成分化均有影响,其中咖啡酸影响最大,对破骨细胞形成分化的抑制作用最强。 Objective To identify effective component in sweet potato extract,and to observe the effect of the effective components on the formation and differentiation of osteoclast.Methods The effective components in sweet potato extracts were identified by using high performance liquid chromatography （HPLC）.The effects of four kinds of effective components in sweet potato extracts on the formation and differentiation of osteoclast were observed by using three different cell culture systems.Results The effective components in sweet potato extracts included coffeic acid,chlorogenic acid,iso-chlorogenic acid and quinic acid.Among the four kinds of effective components,the effect of coffeic acid was most remarkable on the formation and differentiation of osteoclast.The coffeic acid inhibited directly the formation and differentiation of osteoclast,however,the inhibition effect of quinic acid was not obvious.Conclusion The four kinds of effective components in sweet potato extracts can affect the formation and differentiation of osteoclast,and the effect of coffeic acid is the most obvious and its inhibition effect on the formation and differentiation of osteoclast is the strongest.
作者 吴小玉 唐全勇 屈鹏飞 陈彪 杨威 仝金秋 张英怀
机构地区 河北医科大学第二医院口腔颌面外科 河北正定县医院口腔科 河北医科大学第二医院手术室 河北省正定县医院手术室
出处 《河北医药》 CAS 2011年第7期985-987,共3页 Hebei Medical Journal
关键词 甘薯提取物 高效液相色谱 破骨细胞 细胞培养 sweet potato extracts high performance liquid chromatography osteoclast cell culture
分类号 R782.2 [医药卫生—口腔医学]
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参考文献11

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共引文献3

同被引文献25

  • 1吴小玉;贾志宇;唐全勇.甘薯提取物对新生儿大鼠骨生理状态的影响[J]河北医科大学学报,2009(12):1300-1301.
  • 2Gonthier MP,Verny MA,Besson C. Chlorogenic acid bioavailability largely depends on its metabolism by the gut microflora in rats[J].Journal of Nutrition,2003,(06):1853-1859.
  • 3Watanabe T,Kukita T,Kukita A. Direct stimulation of osteoclastogenesis by MIP-1αlpha:evidence obtained from studies using RAW 264 cel clone highly responsive to RANKL[J].Journal of Endocrinology,2004,(01):193-201.
  • 4Krol W,Schel er S,Czuba Z. Inhibition of neutrophils' chemiluminesence by ethanol extract of propolis (EEP) and its phenolic components[J].Journal of Ethnopharmacology,1996,(01):19-25.
  • 5Harrison HF,Peterson JK,Snook ME. Quantity and potential biological activity of caffeic acid in sweet potato [Ipomoea batatas (L.) Lam.] storage root periderm[J].Journal of Agricultural and Food Chemistry,2003,(10):2943-2948.
  • 6Ang ES,Pavlos NJ,Chai LY. Caffeic acid phenethyl ester,an active component of honeybee propolis attenuates osteoclastogenesis and bone resorption via the suppression of RANKL-induced NF-kappaB and NFAT activity[J].Journal of Cellular Physiology,2009,(03):642-649.
  • 7Ha J,Choi HS,Lee Y. Caffeic acid phenethyl ester inhibits osteoclastogenesis by suppressing NF kappaB and downregulating NFATc1 and c-Fos[J].International Journal of Immunopharmacology,2009,(06):774-780.
  • 8Orban Z,Mitsiades N,Burke TR Jr. Caffeic acid phenethyl ester induces leukocyte apoptosis,modulates nuclear factor-kappa B and suppresses acute inflammation[J].Neuroimmunomodulation,2000,(02):99-105.
  • 9Natarajan K,Singh S,Burke TR Jr. Caffeic acid phenethyl ester is a potent and specific inhibitor of activation of nuclear transcription factor NF-κB[J].Proceedings of the National Academy of Sciences(USA),1996,(17):9090-9095.
  • 10Fitzpatrick LR,Wang J,Le T. Caffeic acid phenethyl ester,an inhibitor of nuclear factor-kB,attenuates bacterial peptidoglycan polysaccharide-induced colitis in rats[J].Journal of Pharmacology and Experimental Therapeutics,2001,(03):915-920.

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二级引证文献6

河北医药
2011年 第7期

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