摘要
目的:探讨SNX10过表达对人结直肠癌细胞增殖和EGFR表达的影响。方法:应用基因转染方法将SNX10质粒导入结直肠癌细胞LOVO细胞中,蛋白质印迹法方法鉴定转染后SNX10在细胞中的表达,利用四甲基偶氮唑盐(MTT)法检测SNX10对结直肠癌细胞增殖的影响,利用蛋白质印迹法和免疫荧光法观察EGFR表达量和定位情况。结果:蛋白质印迹法结果显示,SNX10质粒成功导入LOVO细胞中,SNX10的过表达使结直肠癌细胞增殖能力明显降低(F=16.76,P<0.01),EGF刺激后,过表达SNX10的细胞EGFR表达明显低于对照组,荧光定位显示SNX10和EGFR存在共定位关系。结论:SNX10通过调控EGFR表达使结直肠癌细胞增殖能力明显下降,SNX10可能是结直肠癌的增殖抑制基因。
OBJECTIVE:To investigate the effect of sorting nexin 10(SNX10) overexpression on cell proliferation and EGFR expression in colorectal cancer cells.METHODS:SNX10 cDNA was transfected into highly malignant colorectal cancer LOVO cells and stable transfectant clones with high SNX10 expression were established.MTT assay,Western blot and immunofluorescence assay were performed to determine whether SNX10 could have an effect on cell proliferation and EGFR protein expression.RESULTS:SNX10 cDNA was successfully transfected into LOVO cells.The SNX10 transfectant cells exhibited significantly decreased cell proliferative potential in comparision with the control transfectant cells(F=16.76,P0.01).Furthermore,after EGF stimuli,SNX10 colocalised extensively with EGFR and SNX10 overexpression suppressed EGFR expression.CONCLUSION:SNX10 may function as a negative regulator of colorectal cancer cell proliferation by control EGFR expression.
出处
《中华肿瘤防治杂志》
CAS
2010年第21期1734-1737,共4页
Chinese Journal of Cancer Prevention and Treatment
基金
广州市科技支撑项目(2009Z1-E053)