烯效唑对青钱柳试管苗生长及生理特性的影响

Effects of uniconazole on growth and physiological characteristics of Cyclocarya paliurus plantlets

在含0.00(CK)、0.01、0.05、0.10和1.00 mg.L-1烯效唑的WPM培养基上继代培养120 d后,对青钱柳〔Cyclocarya paliurus (Batal.) Iljinskaja〕试管苗的部分生长及生理指标的变化进行了比较研究。结果显示:不同质量浓度烯效唑对青钱柳试管苗的生长及生理指标有不同的影响效应。总体上,随培养基中烯效唑质量浓度的提高,青钱柳试管苗的苗高、叶片数和可溶性蛋白质含量逐渐降低,可溶性糖与可溶性蛋白质含量的比值、SOD和POD活性逐渐提高;在培养基中添加0.01、0.05和0.10 mg.L-1烯效唑对青钱柳试管苗的成活率无显著影响,却可使试管苗的单株鲜质量增加量、叶绿素含量和可溶性糖含量均高于对照;在培养基中添加1.00 mg.L-1烯效唑能显著或极显著降低试管苗的成活率、单株鲜质量增加量、分化芽数、苗高、叶片数以及叶绿素含量、可溶性糖含量和可溶性蛋白质含量,并使苗茎出现异常增粗和矮化。而在含0.10 mg.L-1烯效唑的培养基上,虽然试管苗的苗高、分化芽数和叶片数分别较对照降低了28.03%、9.70%和12.37%,但试管苗的单株鲜质量增加量、叶绿素含量、可溶性糖含量、可溶性糖与可溶性蛋白质含量的比值、SOD和POD活性分别较对照提高了99.39%、14.00%、5.00%、115.43%、129.77%和33.79%。研究结果表明,在培养基中添加0.10 mg.L-1烯效唑可有效改善青钱柳试管苗的生长和生理特性,有效控制苗高和叶片数,促进苗茎的增粗,有助于增强试管苗的抗逆能力。

Changes of some growth and physiological indexes of Cyclocarya paliurus（Batal.） Iljinskaja plantlets were comparatively studied after subcultured for 120 d in WPM medium with 0.00（CK）,0.01,0.05,0.10 and 1.00 mg·L-1 uniconazole.The results show that different concentrations of uniconazole have various influences on growth and physiological indexes of C.paliurus plantlets.Generally,seedling height,leaf number and soluble protein content gradually decrease,and ratio of soluble sugar content to soluble protein content,activities of SOD and POD gradually increase with rising of uniconazole concentration in medium.Adding uniconazole of 0.01,0.05 and 0.10 mg·L-1 in medium has no significant influence on survival rate of plantlets,but leads to higher increment of individual fresh weight and higher contents of chlorophyll and soluble sugar than those in the control.Adding uniconazole of 1.00 mg·L-1 in medium makes significantly or highly significantly decrease of survival rate,individual fresh weight increment,number of differentiated bud,seedling height,leaf number and contents of chlorophyll,soluble sugar and soluble protein,and also makes abnormal thickening and dwarf of stem of plantlets.While,using the medium with 0.10 mg·L-1 uniconazole,although seedling height,number of differentiated bud and leaf number of plantlets decrease by 28.03%,9.70% and 12.37% respectively as compared with those of the control,but individual fresh weight increment,contents of chlorophyll and soluble sugar,ratio of soluble sugar content to soluble protein content,activities of SOD and POD increase by 99.39%,14.00%,5.00%,115.43%,129.77% and 33.79% respectively as compared with those of the control.It is concluded that adding 0.10 mg·L-1 uniconazole in medium can effectively improve growth and physiological characteristics of C.paliurus plantlets,control seedling height and leaf number,promote seedling stem thickening,and help to enhance plantlet＇s stress resistance.