同种异体犬单肺移植过程中的凝血功能

Function changes of blood coagulation in dogs during single lung transplantation

背景:体外循环条件下,肺移植围手术期有凝血和纤溶系统的激活,而对非体外循环下肺移植围手术期凝血功能变化研究较少。目的:检测受体犬单肺移植过程中凝血功能的变化。方法:将健康杂种雄性犬以随机抽签法分成供体和受体两组,行单肺移植,分别于麻醉诱导后20min,再灌注前10min,再灌注后10min,再灌注后60min,采用Sonoclot凝血仪检测受体犬全血激活凝集时间、凝集速率、血小板功能。结果与结论:再灌注后10min全血激活凝集时间数值较麻醉诱导后20min,再灌注前10min,再灌注后60min明显延长(P<0.001)。凝集速率和血小板功能各组之间差异均无显著性意义。说明犬肺移植过程中全血激活凝集时间在再灌注后10min明显延长,再灌注后1h回到正常水平,凝集速率和血小板功能在各个时点变化不大。

BACKGROUND：During the perioperative period of lung transplantation,there is activation of blood coagulation and fibrionlytic system in extracorporeal circulation.But the research addressing function changes of blood coagulation during the perioperative off-pump period of lung transplantation is relatively rare.OBJECTIVE：To detect the function changes of blood coagulation in recipient dogs during single lung transplantation.METHODS：Twenty-four healthy male dogs were randomly divided into donor group and recipient group,followed by single lung transplantation.Activated clotting time（ACT）,clotting rate（CR）,and platelet function（PF） in the recipient group were detected with Sonoclot coagulometer respectively at 20 minutes after anesthesia induction（T1）,10 minutes before reperfusion（T2）,10 minutes after reperfusion（T3） and 60 minutes after reperfusion（T4）.RESULTS AND CONCLUSION：Activated clotting time of T3 was obviously longer than T1,T2,and T4（P 0.001）.CR and PF had no significant changes among the groups.These indicated that during the lung transplantation,activated clotting time was obviously prolonged 10 minutes after reperfusion,and returned to normal level one hour after reperfusion.CR and PF had no obvious changes at different time point.