不同性别肝大部切除小鼠的肝再生

Comparison of hepatic regenerative capacity between male and female mice after partial hepatectomy

背景:各种病因所致的肝硬化及肝癌发病率存在明显的性别差异。肝受损或手术后肝脏均可通过其自身强大的再生能力进行代偿,但肝再生过程中不同性别肝细胞的再生状况是否存在差异尚不清楚。目的:比较肝大部切除后不同性别小鼠肝脏再生能力是否存在差别。方法:采用不同性别成年昆明小鼠行肝脏大部切除,称量手术切除的肝组织,并于术后1,3,5,7,10,14d取再生肝组织,称量后常规制作组织切片备用,取材前2h腹膜下注射5-溴脱氧尿核苷50mg/kg。观察比较不同性别小鼠再生肝组织的结构变化、细胞形态,以及术后各时间点肝再生率、肝组织5-溴脱氧尿核苷的标记指数。结果与结论:苏木精-伊红染色切片镜下结果显示,肝脏大部切除后各时间点不同性别小鼠肝组织结构无明显差别。不同性别小鼠肝大部切除后各时间点肝再生率及5-溴脱氧尿核苷标记指数差异均无显著性意义(P>0.05)。提示肝大部切除后不同性别小鼠肝的再生能力无明显差别。

BACKGROUND：A significant gender difference can be found in the morbidity of liver cirrhosis and hepatoma between male and female.The liver has powerful ability to regenerate after injury or loss of tissue after operation,but whether or not a difference exists in the regeneration and succession of the hepatic cells remain unclear.OBJECTIVE：To compare the hepatic regenerative capacity between male and female mice after partial hepatectomy.METHODS：Adult Kunming mice were subjected to partial hepatectomy.Both the resected and regenerated liver tissues were harvested,weighed,fixed and paraffin embedded for determination of proliferation.The 5-bromo-2-deoxyuridine（BrdU） was injected peritoneal 2 hours before the mice were sacrificed.The liver structure and the morphology of hepatic cells were observed;liver regeneration rate and BrdU labeling index were compared at 1,3,5,7,10 and 14 days after partial hepatectomy.RESULTS AND CONCLUSION：Hematoxylin-eosin staining showed that there was no significant difference in liver structure and the morphology of hepatic cells after partial hepatectomy.No difference could be found in both liver regeneration rate and BrdU labeling index between male and female mice（P 0.05）.There is no significant difference in the capacity of hepatic regeneration after partial hepatectomy between male and female mice.