冻干辐照猪硬脑膜的抗原性

Antigenicity of freeze-dried irradiated pig dura mater

背景:抗原性是异种组织移植最主要的问题,是移植成功与否的关键。目的:观察经冻干辐照处理后的猪硬脑膜抗原性变化。方法:取新鲜猪硬脑膜,经冻干、辐照处理后分别制备成冻干猪硬脑膜、辐照猪硬脑膜和冻干辐照猪硬脑膜。分别移植于大白兔硬脑膜上,于术后3,8周行各组实验动物淋巴细胞毒实验。结果与结论:移植冻干组和辐照组淋巴细胞死亡率比较差异无显著性意义(P>0.05),但均高于冻干辐照组(P<0.05)。结果提示经冻干辐照处理后的猪硬脑膜抗原性低。

BACKGROUND：Antigenicity is the main problem exists in allografts transplantation and decides the fate of transplantation.OBJECTIVE：To observe the antigen changes of freeze-dried irradiated pig dura mater.METHODS：Freeze-dried porcine dura mater,irradiated porcine dura mater and freeze-dried and irradiated porcine dura mater were prepared by freeze-dried and（or） irradiated treatment and were transplanted into dura mater of white rabbits.Immunologic property was determined at 3 and 8 weeks after operation.RESULTS AND CONCLUSION：There was no significant difference of lymphocytes death rates in the freeze-dried group and irradiated group（P 0.05）,both of which were greater than that of the freeze-dried irradiated group（P 0.05）.The results demonstrated that,the pig freeze-dried irradiated dura mater has low antigenicity.