HPLC法测定脑通颗粒中丹酚酸B

Determination of salvianolic acid B in Naotong Granula by HPLC

目的建立HPLC法测定脑通颗粒中丹酚酸B。方法采用迪马色谱柱（250mm×4．6mm，5μm）；以甲醇-乙腈-甲酸-水溶液（30：10：0．5：59．5）为流动相：检测波长286nml体积流量1．0mL／min；柱温40℃；进样量10止。结果丹酚酸B在0．4416-2．2080gg线性关系良好（r=0．9997），样品的平均回收率为98．65％，RSD为1．71％。结论本法重现性好、稳定可靠，可作为脑通颗粒的质量控制方法。

Objective To establish a method for HPLC determination of salvianolic acid B in Naotong Granula. Methods The condition for I-IPLC was Diamonsil Czs column （250mm×4.6mm,5μm） with methanol-acetonitrile-formic acid-water （30 ： 10 ： 0.5 ： 59.5） as the mobile phase. The flow rate was 1.0 mL/min. The column temperature was 40 ＊C. The injection volume was 10 μL, and detection wavelength was 286 nm. Results The linear range was of 0,441 6--2.208 0 μg for salvianolic acid B and the correlation coefficient was 0.999 7. The average recovery was 98.65% with RSD 1.71%. Conclusion The method is reproducible, reliable, and can be used for quality control of Naotong Granula.