头针对脑缺血大鼠唾液酸-神经细胞黏附分子表达的影响

Effect of scalp-acupuncture on expression of PSA-NCAM in acute cerebral ischemia rats

目的研究头针对脑缺血再灌注大鼠唾液酸-神经细胞黏附分子(PSA-NCAM)表达的影响,探讨头针治疗脑缺血病的作用机制。方法采用线栓法制作成大脑中动脉闭阻(MCAO)模型,各组大鼠又按照缺血时间(7 d、14 d、28 d)分为3个亚组,每个时相点10只。头针组动物于栓塞再灌注成功后即行头针治疗,每日1次,直至处死前。各组大鼠处死前1d腹腔注射5-溴脱氧尿核苷(B rdU)液。对各亚组大鼠行神经功能缺损评分(NSS),免疫荧光法计数各组大鼠海马区(DG)B rdU/PSA-NCAM阳性双标细胞。结果第28天头针组的NSS显著低于模型组(P<0.05)。大鼠在缺血再灌注后PSA-NCAM阳性细胞有明显表达,而头针组在不同时相的阳性细胞数量较同时相模型组有明显增加。结论头针能促进B rdU/PSA-NCAM的表达,从而可能诱导神经干细胞(NSCs)的迁移,并能在一定程度上改善神经功能缺损,实现保护脑组织的目的。

Objective It is to research the effect of scalp-acupuncture（SA） on expression of（polysialylated neural cell adhesion molecule,PSA-NCAM） in acute cerebral ischemia/reperfusion injury rats,and to explore the mechanisms of SA in treating for cerebral ischemia.Methods Models of middle cerebral artery occlusion（MCAO） were established by line ligation method,and the models were divided into 3sets respectively according to ischemic time（7 d,14 d,28 d）,and each set was 10.SA rats were treated after MCAO experiments（1 time each day until death）.Each set rats were injected bromodeoxyuridine（BrdU）solution into abdomen.Neurological severity scores（NSS） was applied for each phase point rats.BrdU/PSA-NCAM positive cells of each set were observed with immunofluorescence assay（IFA）.Results On NSS at each phase point： SA group demonstrated obviously declined at 28d comparing with that of model group（P0.05）.On observation of positive cells at each group,model group and SA group significantly increased in contrast to sham group.A clearly difference could be seen on positive cells of SA group comparing with that of model group at each phase point.Conclusion SA can promote the expression of PSA-NCAM and the migration of（neural stem cells,NSCs）,which will be benefit for the neurologic impairment and protect the brain tissue to some extent.