云南省恶性疟原虫裂殖子表面蛋白1基因分型及测序

GENOTYPE AND SEQUENCE ANALYSIS OF MEROZOITE SURFACE PROTEIN 1 OF PLASMODIUM FALCIPARUM ISOLATES IN YUNNAN PROVINCE

目的：确定云南省恶性疟原虫裂殖子表面蛋白１（ Ｍ Ｓ Ｐ １）基因分型和探讨 Ｍ Ｓ Ｐ １ 基因多态性的遗传及地理特征。方法：采用巢式 Ｐ Ｃ Ｒ法和引物标记周期反应测定法，对云南疫区恶性疟原虫群体 Ｍ Ｓ Ｐ１ 基因分型，并对代表株进行基因序列分析。结果：３０ 例云南恶性疟患者，检出３８ 个基因型虫株，其中 Ｍ Ａ Ｄ２０ 型是优势虫株， Ｋ１ 次之， Ｒ Ｏ３３ 最少，并存在不同基因株混合感染现象。扩增片段序列分析表明，云南疫区的 Ｍ Ａ Ｄ２０ 型、 Ｋ１ 型和 Ｒ Ｏ３３型均分别与国际上典型的 Ｍ Ｓ Ｐ１、 Ｍ Ａ Ｄ ２０、 Ｋ１ 和 Ｒ Ｏ ３３ 等位基因代表株具有高度的同源性。结论：以 Ｍ Ｓ Ｐ１ 为基因标记物的基因分型法，有助于掌握流行区疟原虫种群的基因特点、分布及流行特征。

AIM: To identify the genotype of merozoite surface protein 1 of Plasmodium falciparum in Yunnan Province and explore the polymorphism of MSP1 genes in geographical characteristics and genetics. METHODS: Nested polymerase chain reaction was applied to genotyping of P. falciparum isolated in Yunnan Province. Some of parasite alleles were sequenced by dye primer cycle sequencing. RESULTS: In 30 P. falciparum infections, 38 different alleles were found. Of them, the dominant allele was a variant of MAD20, while was K1 less and the RO33 was few. In addition, incidences of mixed allele infections were observed. Sequence analysis showed that DNA sequences of MAD20 , K1 and RO33 alleles from Yunnan were highly homologous with those of international standard strains, respectively. CONCLUSION:Composition and sequence characteristics of P.falciparum arasite population in the endemic area can be detected by genotyping with MSP1 as genetic marker, which would be useful for the prevention and treatment of malaria.