ICAM-1在着床期小鼠PBLC及EC/DC中表达的研究

Study on dynamic changes of ICAM1 in mouse PBLC and EC/DC at periimplantation stage

目的：研究细胞间粘附因子１（ＩＣＡＭ１）在着床期外周血淋巴细胞（ＰＢＬＣ）、子宫内膜及蜕膜细胞（ＥＣ／ＤＣ）中的不同表达特点及动态变化规律。方法：以着床期小鼠为动物模型，细胞分析采用单抗免疫荧光标记及多参数流式细胞术分析技术。结果：发现ＩＣＡＭ１在ＰＢＬＣ、ＥＣ／ＤＣ中的表达均存在明显的动态变化；其中ＩＣＡＭ１的表达在ＰＢＬＣ中于妊娠第２天（Ｄ２）达最低水平，而在ＥＣ／ＤＣ中于妊娠第４天（Ｄ４）降至最低。结论：ＩＣＡＭ１在ＰＢＬＣ及ＥＣ／ＤＣ中的动态表达具有不同的调控机制，这种动态变化参与了着床过程中局部免疫耐受的形成及调控。

Objctive: The study was to investigate the expressions and dynamic changes of intercellular adhesion molecule1(ICAM1) in peripheral blood lymphocytes(PBLC),endometrial and decidual cells(EC/DC) at periimplantation stage. Methods: Cells were stained by direct immunofluorescence staining with FITCconjugated antimouse ICAM1 McAb and studied by multiparameter flow cytometry. Results: Substantial levels of ICAM1 expression were found in PBLC and EC/DC. Both ICAM1 expressions in PBLC and EC/DC had a significant dynamic change during periimplantation stage. The lowest level of ICAM1 expression was on the second day of pregnancy (D2) in PBLC whereas in EC/DC the lowest level was on D4. Conclusion: The expression of ICAM1 in EC/DC was regulated in a manner different from that in PBLC. The dynamic changes of ICAM1 expression may be adapted to accommodate local immune tolerance and could be essential for the regulation of the maternal immune response to the genetically foreing fetus.