摘要
目的:研究细胞间粘附因子1(ICAM1)在着床期外周血淋巴细胞(PBLC)、子宫内膜及蜕膜细胞(EC/DC)中的不同表达特点及动态变化规律。方法:以着床期小鼠为动物模型,细胞分析采用单抗免疫荧光标记及多参数流式细胞术分析技术。结果:发现ICAM1在PBLC、EC/DC中的表达均存在明显的动态变化;其中ICAM1的表达在PBLC中于妊娠第2天(D2)达最低水平,而在EC/DC中于妊娠第4天(D4)降至最低。结论:ICAM1在PBLC及EC/DC中的动态表达具有不同的调控机制,这种动态变化参与了着床过程中局部免疫耐受的形成及调控。
Objctive: The study was to investigate the expressions and dynamic changes of intercellular adhesion molecule1(ICAM1) in peripheral blood lymphocytes(PBLC),endometrial and decidual cells(EC/DC) at periimplantation stage. Methods: Cells were stained by direct immunofluorescence staining with FITCconjugated antimouse ICAM1 McAb and studied by multiparameter flow cytometry. Results: Substantial levels of ICAM1 expression were found in PBLC and EC/DC. Both ICAM1 expressions in PBLC and EC/DC had a significant dynamic change during periimplantation stage. The lowest level of ICAM1 expression was on the second day of pregnancy (D2) in PBLC whereas in EC/DC the lowest level was on D4. Conclusion: The expression of ICAM1 in EC/DC was regulated in a manner different from that in PBLC. The dynamic changes of ICAM1 expression may be adapted to accommodate local immune tolerance and could be essential for the regulation of the maternal immune response to the genetically foreing fetus.
出处
《中国免疫学杂志》
CAS
CSCD
北大核心
1999年第7期312-314,共3页
Chinese Journal of Immunology
基金
中科院动物所生殖生物学国家重点实验室资助