肝癌TIL细胞转导IL-2基因的生物学变化研究

StudyontransferationwithPZIPnewSVIL2genecomplexsintoTILfromhepatocellularcarcinomaandeffectonbiologicalactivitiesoftransferedIL2geneTIL

目的：为探讨ＩＬ２基因导入肝癌浸润淋巴细胞及对其生物学活性的影响。方法：应用逆转录病毒载体介导ＩＬ２基因导入人肝癌浸润淋巴细胞（ＴＩＬ），同时对经转导ＩＬ２基因的肝癌浸润淋巴细胞的杀伤活性，增殖能力及表型等进行了观察。结果：转导ＩＬ２基因后的肝癌浸润淋巴细胞能表达一定量的ＩＬ２，明显高于未转导细胞，但其杀伤肿瘤细胞活性，增殖能力及表型等没有显著变化。结论：应用逆转录病毒载体介导ＩＬ２基因导入人肝癌浸润淋巴细胞获得成功，亦有ＩＬ２基因产物表达。

Objective: TostudyontransferationwithPZIPnewSVIL2genecomplexsintoTILfromhepatocellularcarcinomaandeffectonbiologicalactivitiesoftransferedIL2geneTIL. Methods: Aneukaryoticexpressionvectorcontaininghumaninterleukin2gene(PZIPnewSVIL2)wastransducedintoTILfromhepatocellularcarcinomabyretroviralvectorgenecomplexsandinsametimephenotype,killingactivityandproliferationoftransducedIL2geneTILwereobserved. Results: TransducedIL2geneTILcouldsecretgenerallevelIL2thatwashigherthanthatofTILsecretion,killingactivity,proliferationandphenotypeoftransducedIL2geneTILwerenosignificantchange. Conclusion: ItwassuccessfulthathumanIL2genewastransducedintoTILandTILcellsoftransducedIL2genebegantosecreteIL2after48 h.