转TaCRT基因拟南芥植株表型分析及抗性鉴定

Phenotype Analysis and Resistance Identification of the Transgenic TaCRT Arabidopsis Plants

为了进一步揭示小麦钙网蛋白基因TaCRT是否参与植物对环境胁迫响应,通过根癌农杆菌GV3101介导法将该基因cDNA克隆转入拟南芥,使其异源过量表达,分析转基因拟南芥纯系和野生型植株在表型上的差异,并对转基因株系的抗盐性进行鉴定。结果表明,过表达TaCRT基因的拟南芥纯系植株在正常培养条件下,植株生长相对旺盛,根系较短,分化的不定根数目较多,而在盐胁迫条件下,与野生型相比,转基因株系的耐盐性较强。

In order to further reveal that the wheat calreticulin gene was involved in plant stress response,TaCRT gene was transformed into A.thaliana mediated by Agrobacterium GV3101 by infiltration.The phenotypic differences of transgenic and wild-type Arabidopsis plants were analysed,and salt tolerance of transgenic lines was identified.The results showed that under the normal culture condition,Arabidopsis pure lines of TaCRT gene over-expression grew vigorously,and adventitious root differentiation was strong.Compared to the wild-type Arbidopsis plants,TaCRT-overexpressed Arabidopsis plants displayed more tolerant to NaCl stress.