摘要
目的:CD44基因是一种肿瘤转移相关基因。研究检测CD44v6蛋白在喉癌中的表达及其临床意义。方法:用CD44v6单克隆抗体,通过免疫组化S-P法,对69例喉鳞状细胞癌蜡块标本和10例正常喉粘膜标本进行研究,并结合临床资料进行预后分析。结果:69例喉鳞状细胞癌、10例正常喉粘膜中CD44v6蛋白阳性表达率分别为522%(36/39)和00%(0/10)。CD44v6蛋白阳性表达与肿瘤发生部位、病理扮级和临床分期无相关性(P>005);CD44v6蛋白阳性表达者颈淋巴结转移率(50%,18/36)比阴性表达者高(182%,6/33),两组有显著性差异(P<001)。用Log_rank检验分析CD44v6蛋白表达阴性组生存率高于表达阳性组(P<005)。结论:CD44蛋白的阳性表达与喉鳞癌颈淋巴结转移和生存率显著相关,CD44v6可能作为颈测喉癌转移和预后的一个重要的指标。
Objective: To examine the immunohistochemical
staining of CD44v6 protein in laryngeal squamous cell carcinoma (LSCC) and its clinical
significance. Methods: S_P method of immunohistochemistry with CD44v6 monoclonal
antibody was employed to study the expression of CD44v6 protein in 69 paraffin_embedded
specimens of LSCC and in 10 specimens of normal laryngeal tissues. The clinical pathologic
data and results of follow_up were also analysed. Results: In the 69 cases of LSCC,CD44v6
protein positive rate was 52.2% and in the 10 cases of normal tissues the positive rate was
0.0%.The positive expression of CD44v6 protein did not correlate with the position of tumor,and
the staging and grade of LSCC(P>005);but the expression of CD44v6 protein was higher in
cases with lymph nodal metastasis (50%,18/36) than that in those without lymph nodal
metastasis (18.2%,6/33),and the difference was significant (P<001).By Log_rank test,the
survival rate of CD44v6 negative expression tumors was higher than that of CD44v6 positive
expression tumors (P<0.05). Conclusion: The expression of CDd44v6 is significantly
correlated with lymph nodal metastasis and the survival rate.CD44v6 may have value for
predicting metastasis potential and prognosis.
出处
《中国耳鼻咽喉颅底外科杂志》
CAS
1999年第2期76-79,共4页
Chinese Journal of Otorhinolaryngology-skull Base Surgery
基金
广东省医学科学技术研究计划基金
关键词
CD44基因
肿瘤转移
喉癌
预后
Carcinoma,laryngeal neoplasms\ \
CD44 gene\ \ Metastasis