鼻咽癌活检组织及外周血白细胞EB病毒DNA的检测及其意义

DETECTION OF EPSTEINBARR VIRUS DNAOF LEUCOCYTE AND NASOPHARYNGEALEPITHELIUM IN NASOPHARYNGEAL CARCINOMA

目的：探讨鼻咽组织、外周血白细胞ＥＢＶ基因组分布及与ＮＰＣ的关系。方法：采用ＰＣＲ法检测２６例ＮＰＣ，４例癌旁及１４例慢性鼻咽炎鼻咽组织及外周血白细胞中ＥＢＶＤＮＡ。同时采用酶免疫法测定血清ＶＣＡ－ＩｇＡ。结果：１．ＮＰＣ、癌旁及鼻咽炎组织ＥＢＶＤＮＡ无明显差异。２．ＮＰＣ与鼻咽炎组外周血白细胞ＥＢＶＤＮＡ有明显差异，Ｐ＜０．０５。３．ＮＰＣ与鼻咽炎组血清ＶＣＡ－ＩｇＡ之间存在明显差异。Ｐ＜０．０５。４．同时检测以上三项后三项阳性率及二项阳性率在两组间有显著差异。Ｐ＜０．０５。５．ＮＰＣ活检组织及血ＥＢＶＤＮＡ与血清ＶＣＡ－ＩｇＡ阳性率之间有良好的一致性。结论：ＮＰＣ鼻咽组织存在大量的ＥＢＶＤＮＡ拷贝数，少数分布于外周血白细胞，且与血清ＶＣＡ－ＩｇＡ有良好的一致性。３项套式检测比单项鼻咽活检组织ＥＢＶＤＮＡ或血清ＶＣＡ－ＩｇＡ更有助于对ＮＰＣ的诊断。

Objective: To study the EBV DNA distribution in the human body and the relationship between EBV DNA and NPC. Methods: EBV DNA was detected in 26 cases of NPC, 4 cases of epithelium adjacent to NPC and 14 cases of chronic nasopharyngitis (CNPG) with polymerase chain reaction (PCR). At the same time, serum VCAIgA was also measured in NPC and CNPG with enzyme-immunoassay. Results: The positive rate of NPC EBV DNA in the three groups was not significantly different, but there was a remarkble difference in leucocyte EBV DNA and serum VCAIgA between NPC and CNPG(P<0.05). It was also found that the positive rates of multiparameters including serum VCAIgA and EBV DNA both in leucocytes and NPE in NPC were higher than those in CNPG and there was a good consistency between EBV DNA in NPC or serum leucocyte and serum VCAIgA. But NPE EBV DNA was not relative to the stage and lymph node involvement in NPC. Conclusion: There are many EBV DNA copies in NPE or leucocytes of NPC and there is also a good consistency between EBV DNA in NPE or leucocyte and serum VCAIgA. So it will be more helpful to NPC diagnosis if a combination of the above three assays is taken.